Department of Cardiac Surgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.
Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Ministry of Science & Technology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.
Medicine (Baltimore). 2024 Apr 19;103(16):e37616. doi: 10.1097/MD.0000000000037616.
Atherosclerosis is a chronic, progressive vascular disease. The relationship between CASP1 gene expression and atherosclerosis remains unclear. The atherosclerosis dataset GSE132651 and GSE202625 profiles were downloaded from gene expression omnibus. Differentially expressed genes (DEGs) were screened. The construction and analysis of protein-protein interaction network, functional enrichment analysis, gene set enrichment analysis, and Comparative Toxicogenomics Database analysis were performed. Gene expression heatmap was drawn. TargetScan was used to screen miRNAs that regulate central DEG. 47 DEGs were identified. According to gene ontology analysis, they were mainly enriched in the regulation of stimulus response, response to organic matter, extracellular region, extracellular region, and the same protein binding. Kyoto Encyclopedia of Gene and Genome analysis results showed that the target cells were mainly enriched in the PI3K-Akt signaling pathway, Ras signaling pathway, and PPAR signaling pathway. In the enrichment project of Metascape, vascular development, regulation of body fluid levels, and positive regulation of cell motility can be seen in the gene ontology enrichment project. Eleven core genes (CASP1, NLRP3, MRC1, IRS1, PPARG, APOE, IL13, FGF2, CCR2, ICAM1, HIF1A) were obtained. IRS1, PPARG, APOE, FGF2, CCR2, and HIF1A genes are identified as core genes. Gene expression heatmap showed that CASP1 was highly expressed in atherosclerosis samples and low expressed in normal samples. NLRP3, MRC1, IRS1, PPARG, APOE, IL13, FGF2, CCR2, ICAM1, HIF1A were low expressed in atherosclerosis samples. CTD analysis showed that 5 genes (CASP1, NLRP3, CCR2, ICAM1, HIF1A) were found to be associated with pneumonia, inflammation, cardiac enlargement, and tumor invasiveness. CASP1 gene is highly expressed in atherosclerosis. The higher the CASP1 gene, the worse the prognosis.
动脉粥样硬化是一种慢性、进行性血管疾病。CASP1 基因表达与动脉粥样硬化的关系尚不清楚。从基因表达综合数据库中下载了 GSE132651 和 GSE202625 数据集。筛选差异表达基因（DEGs）。构建和分析蛋白质-蛋白质相互作用网络、功能富集分析、基因集富集分析和比较毒理学基因组数据库分析。绘制基因表达热图。使用 TargetScan 筛选调节核心 DEG 的 miRNAs。鉴定出 47 个 DEG。根据基因本体论分析，它们主要富集在刺激反应、对有机物的反应、细胞外区、细胞外区和相同蛋白结合的调节中。京都基因与基因组百科全书分析结果表明，靶细胞主要富集在 PI3K-Akt 信号通路、Ras 信号通路和 PPAR 信号通路中。在 Metascape 的富集项目中，可以看到基因本体论富集项目中的血管发育、体液水平调节和细胞运动的正调节。获得了 11 个核心基因（CASP1、NLRP3、MRC1、IRS1、PPARG、APOE、IL13、FGF2、CCR2、ICAM1、HIF1A）。IRS1、PPARG、APOE、FGF2、CCR2 和 HIF1A 基因被鉴定为核心基因。基因表达热图显示，CASP1 在动脉粥样硬化样本中高表达，在正常样本中低表达。NLRP3、MRC1、IRS1、PPARG、APOE、IL13、FGF2、CCR2、ICAM1 在动脉粥样硬化样本中低表达。CTD 分析表明，有 5 个基因（CASP1、NLRP3、CCR2、ICAM1、HIF1A）与肺炎、炎症、心脏增大和肿瘤侵袭性有关。CASP1 基因在动脉粥样硬化中高表达。CASP1 基因越高，预后越差。
