State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Vegetable Biology, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China.
J Hazard Mater. 2024 Jun 5;471:134276. doi: 10.1016/j.jhazmat.2024.134276. Epub 2024 Apr 10.
Environmental pollution from cadmium (Cd) presents a serious threat to plant growth and development. Therefore, it's crucial to find out how plants resist this toxic metal to develop strategies for remediating Cd-contaminated soils. In this study, we identified CIP1, a transporter protein, by screening interactors of the protein kinase CIPK23. CIP1 is located in vesicles membranes and can transport Cd when expressed in yeast cells. Cd stress specifically induced the accumulation of CIP1 transcripts and functional proteins, particularly in the epidermal cells of the root tip. CIKP23 could interact directly with the central loop region of CIP1, phosphorylating it, which is essential for the efficient transport of Cd. A loss-of-function mutation of CIP1 in wild-type plants led to increased sensitivity to Cd stress. Conversely, tobacco plants overexpressing CIP1 exhibited improved Cd tolerance and increased Cd accumulation capacity. Interestingly, this Cd accumulation was restricted to roots but not shoots, suggesting that manipulating CIP1 does not risk Cd contamination of plants' edible parts. Overall, this study characterizes a novel Cd transporter, CIP1, with potential to enhance plant tolerance to Cd toxicity while effectively eliminating environmental contamination without economic losses.
镉（Cd）污染对植物的生长和发育构成了严重威胁。因此，了解植物如何抵抗这种有毒金属对于开发修复 Cd 污染土壤的策略至关重要。在这项研究中，我们通过筛选蛋白激酶 CIPK23 的相互作用蛋白，鉴定出一种转运蛋白 CIP1。CIP1 位于液泡膜中，在酵母细胞中表达时可以运输 Cd。Cd 胁迫特异性地诱导 CIP1 转录本和功能蛋白的积累，特别是在根尖的表皮细胞中。CIPK23 可以与 CIP1 的中心环区域直接相互作用，磷酸化 CIP1，这对于 Cd 的有效运输是必不可少的。在野生型植物中 CIP1 的功能丧失突变导致对 Cd 胁迫的敏感性增加。相反，过表达 CIP1 的烟草植物表现出对 Cd 胁迫的耐受性提高和 Cd 积累能力增强。有趣的是，这种 Cd 积累仅限于根部，而不在地上部分，这表明操纵 CIP1 不会增加植物可食用部分的 Cd 污染风险。总体而言，这项研究描述了一种新型的 Cd 转运蛋白 CIP1，它具有增强植物对 Cd 毒性的耐受性的潜力，同时有效地消除环境污染，而不会造成经济损失。
