Department of Biology, Faculty of Sciences, Mersin University, Mersin, 33343, Turkey.
Department of Agricultural Biotechnology, Seyrani Agriculture Faculty, Erciyes University, Nutral Therapy Co., Erciyes Technopark, Kayseri, 38039, Turkey.
Mol Biol Rep. 2024 Apr 20;51(1):559. doi: 10.1007/s11033-024-09493-7.
Methylprednisolone (MP) is a pharmaceutical agent employed in the management of Leukemia, which is a systemic malignancy that arises from abnormalities in the hematological system. Numerous investigations in the field of cancer research have directed their attention towards propolis, a natural substance with significant potential as a treatment-supportive agent. Its utilization aims to mitigate the potential adverse effects associated with chemotherapy medications. The objective of this study was to examine the impact of olive oil-based propolis (OEP) and caffeic acid phenethyl ester (CAPE) on the treatment of acute myeloid leukemia, as well as to determine if they exhibit a synergistic effect when combined with the therapeutic support product methylprednisolone.
The proliferation of HL-60 cells was quantified using the WST-8 kit. The PI Staining technique was employed to do cell cycle analysis of DNA in cells subjected to OEP, CAPE, and MP, with subsequent measurement by flow cytometry. The apoptotic status of cells was determined by analyzing them using flow cytometry after staining with the Annexin V-APC kit. The quantification of apoptotic gene expression levels was conducted in HL-60 cells. In HL-60 cells, the IC dosages of CAPE and MP were determined to be 1 × 10 M and 5 × 10 M, respectively. The HL-60 cells were subjected to apoptosis and halted in the G/G and G/M phases of the cell cycle after being treated with MP, CAPE, and OEP.
Propolis and its constituents have the potential to serve as effective adjunctive therapies in chemotherapy.
甲泼尼龙（MP）是一种用于治疗白血病的药物，白血病是一种起源于血液系统异常的全身性恶性肿瘤。癌症研究领域的许多研究都将注意力集中在具有治疗支持作用的天然物质蜂胶上。它的使用旨在减轻化疗药物可能产生的不良反应。本研究旨在探讨橄榄油基蜂胶（OEP）和咖啡酸苯乙酯（CAPE）对急性髓细胞白血病的治疗作用，并确定它们与治疗支持产品甲泼尼龙联合使用是否具有协同作用。
使用 WST-8 试剂盒定量测定 HL-60 细胞的增殖。采用 PI 染色技术对 OEP、CAPE 和 MP 处理的细胞进行 DNA 细胞周期分析，然后通过流式细胞术进行测量。通过用 Annexin V-APC 试剂盒对细胞进行染色，用流式细胞术分析细胞的凋亡状态。在 HL-60 细胞中进行凋亡基因表达水平的定量。在 HL-60 细胞中，CAPE 和 MP 的 IC 剂量分别为 1×10 M 和 5×10 M。MP、CAPE 和 OEP 处理后，HL-60 细胞发生凋亡，并停滞在细胞周期的 G/G 和 G/M 期。
蜂胶及其成分有可能成为化疗的有效辅助治疗方法。
