Zhou Caibi, Tao Fang, Long Rupiao, Yang Xiaoting, Wu Xingli, Xiang Lan, Zhou Xiaolu, Girdthai Teerayoot
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun, 558000, China.
Sci Rep. 2024 Apr 21;14(1):9131. doi: 10.1038/s41598-024-55010-y.
The chloroplast (cp) genome sequence of Mussaenda pubescens, a promising resource that is used as a traditional medicine and drink, is important for understanding the phylogenetic relationships among the Mussaenda family and genetic improvement and reservation. This research represented the first comprehensive description of the morphological characteristics of M. pubescens, as well as an analysis of the complete cp genome and phylogenetic relationship. The results indicated a close relationship between M. pubescens and M. hirsutula based on the morphological characteristics of the flower and leaves. The cp was sequenced using the Illumina NovaSeq 6000 platform. The results indicated the cp genome of M. pubescens spanned a total length of 155,122 bp, including a pair of inverted repeats (IRA and IRB) with a length of 25,871 bp for each region, as well as a large single-copy (LSC) region and a small single-copy (SSC) region with lengths of 85,370 bp and 18,010 bp, respectively. The results of phylogenetic analyses demonstrated that species within the same genus displayed a tendency to group closely together. It was suggested that Antirhea, Cinchona, Mitragyna, Neolamarckia, and Uncaria might have experienced an early divergence. Furthermore, M. hirsutula showed a close genetic connection to M. pubescens, with the two species having partially overlapping distributions in China. This study presents crucial findings regarding the identification, evolution, and phylogenetic research on Mussaenda plants, specifically targeting M. pubescens.
作为一种被用作传统药物和饮品的有前景资源，白花龙船花的叶绿体（cp）基因组序列对于理解龙船花科植物之间的系统发育关系以及遗传改良和保存具有重要意义。本研究首次全面描述了白花龙船花的形态特征，并对其完整的cp基因组和系统发育关系进行了分析。结果表明，基于花和叶的形态特征，白花龙船花与粗毛龙船花关系密切。使用Illumina NovaSeq 6000平台对cp进行了测序。结果表明，白花龙船花的cp基因组全长155,122 bp，包括一对长度均为25,871 bp的反向重复序列（IRA和IRB），以及长度分别为85,370 bp和18,010 bp的大单拷贝（LSC）区域和小单拷贝（SSC）区域。系统发育分析结果表明，同一属内的物种倾向于紧密聚类。研究表明，鱼骨木属、金鸡纳属、帽柱木属、新乌檀属和钩藤属可能经历了早期分化。此外，粗毛龙船花与白花龙船花显示出密切的遗传联系，这两个物种在中国部分地区分布重叠。本研究提供了关于龙船花属植物，特别是白花龙船花的鉴定、进化和系统发育研究的关键发现。
