Otero Clara Isabel Anton Y, Marger Laurine, di Bella Enrico, Feilzer Albert, Krejci Ivo, Abdelaziz Marwa
Division of Cariology and Endodontology, CUMD - University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Department of Dental Material Sciences, Academic Center for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands.
Biomater Investig Dent. 2024 Apr 5;11:40311. doi: 10.2340/biid.v11.40311. eCollection 2024.
To evaluate the penetration of a dye in root dentin after activation with different laser wavelengths.
Palatal roots of 38 human molars were enlarged and disinfected. Irrigation activation was performed with an Er:YAG laser: @50 mJ, 15 Hz (Er:YAG); a 9.3 µm CO laser: @40% power (CO); diode lasers 455 nm/970 nm: @0.8 W, 15 Hz (D455, D970) and 808/980 nm: @1 W (D808, D980) and compared to positive control: etching with 35% HPO (POS); negative control: water (NEG) and conventional needle irrigation: NaOCl and ethylenediaminetetraacetic acid (EDTA) (CONV). Methylene blue solution was introduced in the canal and laser-activated or left untouched for 100 s before the roots were dried and cut into horizontal slices. Dye penetration was automatically calculated by color recognition of two samples per root third ( = 8 per group in each coronal, middle and apical root thirds). The presence and absence of a smear layer was checked in two additional samples of the negative and positive control under scanning electron microscopy (SEM).
Full-depth infiltration was not achieved in any group. Dye penetration in CONV was significantly less than in Er:YAG, CO POS, D455, D970, D808 and similar to NEG and D980 when results of different root thirds were pooled.
Laser activation using certain parameters enhanced dye penetration compared to conventional needle irrigation with NaOCl and EDTA (CONV).
评估不同激光波长激活后染料在牙根牙本质中的渗透情况。
选取38颗人类磨牙的腭根进行扩大和消毒。使用铒激光（Er:YAG）：50 mJ，15 Hz（Er:YAG）；9.3 µm二氧化碳激光（CO）：40%功率（CO）；455 nm/970 nm二极管激光：0.8 W，15 Hz（D455、D970）以及808/980 nm二极管激光：1 W（D808、D980）进行冲洗激活，并与阳性对照：用35%磷酸（POS）酸蚀；阴性对照：用水（NEG）以及传统针管冲洗：次氯酸钠和乙二胺四乙酸（EDTA）（CONV）进行比较。将亚甲蓝溶液注入根管，激光激活或静置100秒后，将牙根干燥并切成水平切片。通过对每个牙根三分之一处的两个样本进行颜色识别自动计算染料渗透情况（每组在牙冠、牙根中部和根尖三分之一处各8个样本）。在扫描电子显微镜（SEM）下对阴性和阳性对照的另外两个样本检查有无玷污层。
所有组均未实现全层渗透。当汇总不同牙根三分之一处的结果时，CONV组的染料渗透明显少于Er:YAG、CO、POS、D455、D970、D808组，且与NEG和D980组相似。
与使用次氯酸钠和乙二胺四乙酸（EDTA）的传统针管冲洗（CONV）相比，使用特定参数的激光激活可增强染料渗透。
