• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

肌腱仿生电纺 PLGA 纤维束诱导羊膜上皮干细胞早期上皮-间充质转化和肌腱分化。

Tendon Biomimetic Electrospun PLGA Fleeces Induce an Early Epithelial-Mesenchymal Transition and Tenogenic Differentiation on Amniotic Epithelial Stem Cells.

机构信息

Unit of Basic and Applied Biosciences, Faculty of Bioscience and Agro-Food and Environmental Technology, University of Teramo, 64100 Teramo, Italy.

Laboratory of Bacteriology, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "Giuseppe Caporale", 64100 Teramo, Italy.

出版信息

Cells. 2020 Jan 27;9(2):303. doi: 10.3390/cells9020303.

DOI:10.3390/cells9020303
PMID:32012741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7072418/
Abstract

The design of tendon biomimetic electrospun fleece with Amniotic Epithelial Stem Cells (AECs) that have shown a high tenogenic attitude may represent an alternative strategy to overcome the unsatisfactory results of conventional treatments in tendon regeneration. In this study, we evaluated AEC-engineered electrospun poly(lactide-co-glycolide) (PLGA) fleeces with highly aligned fibers (ha-PLGA) that mimic tendon extracellular matrix, their biocompatibility, and differentiation towards the tenogenic lineage. PLGA fleeces with randomly distributed fibers (rd-PLGA) were generated as control. Optimal cell infiltration and biocompatibility with both PLGA fleeces were shown. However, only ha-PLGA fleeces committed AECs towards an Epithelial-Mesenchymal Transition (EMT) after 48 h culture, inducing their cellular elongation along the fibers' axis and the upregulation of mesenchymal markers. AECs further differentiated towards tenogenic lineage as confirmed by the up-regulation of tendon-related genes and Collagen Type 1 (COL1) protein expression that, after 28 days culture, appeared extracellularly distributed along the direction of ha-PLGA fibers. Moreover, long-term co-cultures of AEC-ha-PLGA bio-hybrids with fetal tendon explants significantly accelerated of half time AEC tenogenic differentiation compared to ha-PLGA fleeces cultured only with AECs. The fabricated tendon biomimetic ha-PLGA fleeces induce AEC tenogenesis through an early EMT, providing a potential tendon substitute for tendon engineering research.

摘要

具有高肌腱形成倾向的羊膜上皮干细胞（AECs）构建的肌腱仿生电纺纤维毡可能代表了克服肌腱再生中传统治疗方法不满意结果的另一种策略。在这项研究中，我们评估了具有高度取向纤维（ha-PLGA）的 AEC 工程化电纺聚乳酸-共-乙醇酸（PLGA）纤维毡，其模仿了肌腱细胞外基质及其生物相容性和向肌腱谱系分化的能力。生成了具有随机分布纤维的 PLGA 纤维毡（rd-PLGA）作为对照。结果显示，两种 PLGA 纤维毡均具有最佳的细胞渗透和生物相容性。然而，只有 ha-PLGA 纤维毡在 48 小时培养后促使 AEC 发生上皮-间充质转化（EMT），诱导其沿着纤维轴的细胞伸长并上调间充质标志物。进一步证实 AEC 向肌腱谱系分化，通过上调肌腱相关基因和胶原蛋白 1（COL1）蛋白表达。在 28 天培养后，COL1 蛋白沿着 ha-PLGA 纤维的方向分布在细胞外。此外，AEC-ha-PLGA 生物杂种与胎儿肌腱外植体的长期共培养与仅用 AEC 培养 ha-PLGA 纤维毡相比，显著加速了 AEC 肌腱形成的一半时间。构建的肌腱仿生 ha-PLGA 纤维毡通过早期 EMT 诱导 AEC 肌腱形成，为肌腱工程研究提供了一种潜在的肌腱替代物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/fde1d78105d0/cells-09-00303-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/457e8cd00897/cells-09-00303-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/61bfc04805d4/cells-09-00303-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/0f86c4ae17d4/cells-09-00303-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/39a5960fbbad/cells-09-00303-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/1a4f09bb9e6e/cells-09-00303-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/225d31fa9b5d/cells-09-00303-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/b28d3af9a48e/cells-09-00303-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/fde1d78105d0/cells-09-00303-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/457e8cd00897/cells-09-00303-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/61bfc04805d4/cells-09-00303-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/0f86c4ae17d4/cells-09-00303-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/39a5960fbbad/cells-09-00303-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/1a4f09bb9e6e/cells-09-00303-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/225d31fa9b5d/cells-09-00303-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/b28d3af9a48e/cells-09-00303-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06c6/7072418/fde1d78105d0/cells-09-00303-g008.jpg

相似文献

1
Tendon Biomimetic Electrospun PLGA Fleeces Induce an Early Epithelial-Mesenchymal Transition and Tenogenic Differentiation on Amniotic Epithelial Stem Cells.肌腱仿生电纺 PLGA 纤维束诱导羊膜上皮干细胞早期上皮-间充质转化和肌腱分化。
Cells. 2020 Jan 27;9(2):303. doi: 10.3390/cells9020303.
2
Electrospun PLGA Fiber Diameter and Alignment of Tendon Biomimetic Fleece Potentiate Tenogenic Differentiation and Immunomodulatory Function of Amniotic Epithelial Stem Cells.静电纺丝 PLGA 纤维直径和肌腱仿生绒毡层的对齐方式增强了羊膜上皮干细胞的腱向分化和免疫调节功能。
Cells. 2020 May 13;9(5):1207. doi: 10.3390/cells9051207.
3
Fabrication and Plasma Surface Activation of Aligned Electrospun PLGA Fiber Fleeces with Improved Adhesion and Infiltration of Amniotic Epithelial Stem Cells Maintaining their Teno-inductive Potential.聚乳酸-羟基乙酸共聚物纤维毡的定向静电纺丝制备及其等离子体表面活化以提高羊膜上皮干细胞的黏附与浸润能力并保持其肌腱诱导潜能。
Molecules. 2020 Jul 11;25(14):3176. doi: 10.3390/molecules25143176.
4
Amniotic Epithelial Stem Cells Counteract Acidic Degradation By-Products of Electrospun PLGA Scaffold by Improving Their Immunomodulatory Profile In Vitro.羊膜上皮干细胞通过改善其体外免疫调节特性来对抗电纺聚乳酸-羟基乙酸共聚物支架的酸性降解副产物。
Cells. 2021 Nov 18;10(11):3221. doi: 10.3390/cells10113221.
5
Indirect co-culture with tendons or tenocytes can program amniotic epithelial cells towards stepwise tenogenic differentiation.与肌腱或肌腱细胞间接共培养可以将羊膜上皮细胞向逐步肌腱分化的方向进行编程。
PLoS One. 2012;7(2):e30974. doi: 10.1371/journal.pone.0030974. Epub 2012 Feb 10.
6
Collagen and chondroitin sulfate functionalized bioinspired fibers for tendon tissue engineering application.胶原和硫酸软骨素功能化仿生纤维在肌腱组织工程中的应用。
Int J Biol Macromol. 2021 Feb 15;170:248-260. doi: 10.1016/j.ijbiomac.2020.12.152. Epub 2020 Dec 29.
7
An epigenetic bioactive composite scaffold with well-aligned nanofibers for functional tendon tissue engineering.具有良好取向纳米纤维的表观遗传生物活性复合支架用于功能性腱组织工程。
Acta Biomater. 2018 Jan 15;66:141-156. doi: 10.1016/j.actbio.2017.09.036. Epub 2017 Sep 28.
8
Electrospun thymosin Beta-4 loaded PLGA/PLA nanofiber/ microfiber hybrid yarns for tendon tissue engineering application.静电纺丝载胸腺肽β-4 的 PLGA/PLA 纳米纤维/微纤维混合纱线在肌腱组织工程中的应用。
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110268. doi: 10.1016/j.msec.2019.110268. Epub 2019 Oct 12.
9
Hydroxyapatite/collagen coating on PLGA electrospun fibers for osteogenic differentiation of bone marrow mesenchymal stem cells.PLGA 电纺纤维上的羟基磷灰石/胶原涂层对骨髓间充质干细胞成骨分化的影响。
J Biomed Mater Res A. 2018 Nov;106(11):2863-2870. doi: 10.1002/jbm.a.36475. Epub 2018 Oct 5.
10
Amniotic epithelial stem cell biocompatibility for electrospun poly(lactide-co-glycolide), poly(ε-caprolactone), poly(lactic acid) scaffolds.羊膜上皮干细胞对电纺聚（丙交酯-共-乙交酯）、聚（己内酯）、聚乳酸支架的生物相容性。
Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:321-9. doi: 10.1016/j.msec.2016.06.092. Epub 2016 Jul 1.

引用本文的文献

1
Fibrin Scaffolds Perfused with Transforming Growth Factor-β1 as an In Vitro Model to Study Healthy and Tendinopathic Human Tendon Stem/Progenitor Cells.转化生长因子-β1 灌注纤维蛋白支架作为体外模型研究健康和腱病人类肌腱干/祖细胞。
Int J Mol Sci. 2024 Sep 3;25(17):9563. doi: 10.3390/ijms25179563.
2
Mechanobiological Strategies to Enhance Ovine () Adipose-Derived Stem Cells Tendon Plasticity for Regenerative Medicine and Tissue Engineering Applications.用于再生医学和组织工程应用的增强绵羊脂肪来源干细胞肌腱可塑性的机械生物学策略。
Animals (Basel). 2024 Jul 31;14(15):2233. doi: 10.3390/ani14152233.
3
Assessing the functional potential of conditioned media derived from amniotic epithelial stem cells engineered on 3D biomimetic scaffolds: An in vitro model for tendon regeneration.

本文引用的文献

1
Fibrinogen nanofibers for guiding endothelial cell behavior.用于引导内皮细胞行为的纤维蛋白原纳米纤维。
Biomater Sci. 2013 Oct 28;1(10):1065-1073. doi: 10.1039/c3bm60124b. Epub 2013 Jul 8.
2
Electrospun PCL/Gel-aligned scaffolds enhance the biomechanical strength in tendon repair.静电纺丝的 PCL/凝胶对齐支架增强了肌腱修复中的生物力学强度。
J Mater Chem B. 2019 Aug 7;7(31):4801-4810. doi: 10.1039/c9tb00837c.
3
Effect of Chemical Composition Variant and Oxygen Plasma Treatments on the Wettability of PLGA Thin Films, Synthesized by Direct Copolycondensation.
评估在3D仿生支架上工程化的羊膜上皮干细胞衍生的条件培养基的功能潜力：一种用于肌腱再生的体外模型。
Mater Today Bio. 2024 Feb 18;25:101001. doi: 10.1016/j.mtbio.2024.101001. eCollection 2024 Apr.
4
An overview of the material science and knowledge of nanomedicine, bioscaffolds, and tissue engineering for tendon restoration.肌腱修复的材料科学以及纳米医学、生物支架和组织工程知识概述。
Front Bioeng Biotechnol. 2023 Jun 14;11:1199220. doi: 10.3389/fbioe.2023.1199220. eCollection 2023.
5
Recent advances in tendon tissue engineering strategy.肌腱组织工程策略的最新进展。
Front Bioeng Biotechnol. 2023 Feb 20;11:1115312. doi: 10.3389/fbioe.2023.1115312. eCollection 2023.
6
Tendon 3D Scaffolds Establish a Tailored Microenvironment Instructing Paracrine Mediated Regenerative Amniotic Epithelial Stem Cells Potential.肌腱3D支架构建了一个定制的微环境，引导旁分泌介导的再生羊膜上皮干细胞潜能。
Biomedicines. 2022 Oct 14;10(10):2578. doi: 10.3390/biomedicines10102578.
7
Polylactide Perspectives in Biomedicine: From Novel Synthesis to the Application Performance.生物医学中的聚乳酸研究进展：从新型合成到应用性能
Pharmaceutics. 2022 Aug 11;14(8):1673. doi: 10.3390/pharmaceutics14081673.
8
Biomaterials for Regenerative Medicine in Italy: Brief State of the Art of the Principal Research Centers.意大利再生医学用生物材料：主要研究中心的简要现状概述。
Int J Mol Sci. 2022 Jul 26;23(15):8245. doi: 10.3390/ijms23158245.
9
Advanced Nanofiber-Based Scaffolds for Achilles Tendon Regenerative Engineering.用于跟腱再生工程的先进纳米纤维基支架
Front Bioeng Biotechnol. 2022 Jun 30;10:897010. doi: 10.3389/fbioe.2022.897010. eCollection 2022.
10
Tendon Healing Response Is Dependent on Epithelial-Mesenchymal-Tendon Transition State of Amniotic Epithelial Stem Cells.肌腱愈合反应取决于羊膜上皮干细胞的上皮-间充质-肌腱转化状态。
Biomedicines. 2022 May 19;10(5):1177. doi: 10.3390/biomedicines10051177.
化学成分变化及氧等离子体处理对直接共缩聚合成的聚乳酸-羟基乙酸共聚物（PLGA）薄膜润湿性的影响
Polymers (Basel). 2018 Oct 12;10(10):1132. doi: 10.3390/polym10101132.
4
Collagen Fiber Array of Peritumoral Stroma Influences Epithelial-to-Mesenchymal Transition and Invasive Potential of Mammary Cancer Cells.肿瘤周围基质的胶原纤维阵列影响乳腺癌细胞的上皮-间质转化和侵袭潜能。
J Clin Med. 2019 Feb 7;8(2):213. doi: 10.3390/jcm8020213.
5
Functional regeneration of tendons using scaffolds with physical anisotropy engineered via microarchitectural manipulation.利用微观结构操控工程化具有物理各向异性的支架实现肌腱的功能再生。
Sci Adv. 2018 Oct 19;4(10):eaat4537. doi: 10.1126/sciadv.aat4537. eCollection 2018 Oct.
6
Biomaterials in Tendon and Skeletal Muscle Tissue Engineering: Current Trends and Challenges.肌腱和骨骼肌组织工程中的生物材料：当前趋势与挑战
Materials (Basel). 2018 Jun 29;11(7):1116. doi: 10.3390/ma11071116.
7
Dual-layer aligned-random nanofibrous scaffolds for improving gradient microstructure of tendon-to-bone healing in a rabbit extra-articular model.双层定向随机纳米纤维支架改善兔关节外模型腱骨愈合的梯度微结构。
Int J Nanomedicine. 2018 Jun 18;13:3481-3492. doi: 10.2147/IJN.S165633. eCollection 2018.
8
Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance.家畜胎盘干细胞：转化潜力与临床相关性。
Cell Transplant. 2018 Jan;27(1):93-116. doi: 10.1177/0963689717724797.
9
Melt electrowriting below the critical translation speed to fabricate crimped elastomer scaffolds with non-linear extension behaviour mimicking that of ligaments and tendons.在临界翻译速度以下进行熔融电写入，以制造出具有类似于韧带和肌腱的非线性延伸行为的卷曲弹性体支架。
Acta Biomater. 2018 May;72:110-120. doi: 10.1016/j.actbio.2018.03.023. Epub 2018 Mar 17.
10
Effect of scaffold morphology and cell co-culture on tenogenic differentiation of HADMSC on centrifugal melt electrospun poly (L‑lactic acid) fibrous meshes.支架形态和细胞共培养对离心熔融静电纺丝聚（L-乳酸）纤维网中 HADMSC 腱向分化的影响。
Biofabrication. 2017 Nov 14;9(4):044106. doi: 10.1088/1758-5090/aa8fb8.