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牛用减毒和标记牛疱疹病毒1型联合疫苗的安全性及保护效力研究。

Investigation of the safety and protective efficacy of an attenuated and marker -BoHV-1 combined vaccine in bovines.

作者信息

Zhang Sen, Liu Guoxing, Zhang Yisheng, Wang Chen, Xu Xiaowen, Zhao Yuhao, Xiang Zhijie, Wu Wenying, Yang Li, Chen Jianguo, Guo Aizhen, Chen Yingyu

机构信息

State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.

出版信息

Front Immunol. 2024 Apr 4;15:1367253. doi: 10.3389/fimmu.2024.1367253. eCollection 2024.

DOI:10.3389/fimmu.2024.1367253
PMID:38646533
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11027501/
Abstract

Bovine respiratory disease (BRD) is one of the most common diseases in the cattle industry worldwide; it is caused by multiple bacterial or viral coinfections, of which () and bovine herpesvirus type 1 (BoHV-1) are the most notable pathogens. Although live vaccines have demonstrated better efficacy against BRD induced by both pathogens, there are no combined live and marker vaccines. Therefore, we developed an attenuated and marker -BoHV-1 combined vaccine based on the HB150 and BoHV-1 gG-/tk- strain previously constructed in our lab and evaluated in rabbits. This study aimed to further evaluate its safety and protective efficacy in cattle using different antigen ratios. After immunization, all vaccinated cattle had a normal rectal temperature and mental status without respiratory symptoms. CD4, CD8, and CD19 cells significantly increased in immunized cattle and induced higher humoral and cellular immune responses, and the expression of key cytokines such as IL-4, IL-12, TNF-α, and IFN-γ can be promoted after vaccination. The 1.0 × 10 CFU of HB150 and 1.0 × 10 TCID BoHV-1 gG-/tk- combined strain elicited the most antibodies while significantly increasing IgG and cellular immunity after challenge. In conclusion, the HB150 and BoHV-1 gG-/tk- combined strain was clinically safe and protective in calves; the mix of 1.0 × 10 CFU of HB150 and 1.0 × 10 TCID BoHV-1 gG-/tk- strain was most promising due to its low amount of shedding and highest humoral and cellular immune responses compared with others. This study introduces an -BoHV-1 combined vaccine for application in the cattle industry.

摘要

牛呼吸道疾病（BRD）是全球养牛业中最常见的疾病之一；它由多种细菌或病毒混合感染引起，其中（）和牛疱疹病毒1型（BoHV-1）是最主要的病原体。尽管活疫苗已证明对这两种病原体引起的BRD具有更好的疗效，但目前尚无联合活疫苗和标记疫苗。因此，我们基于之前在本实验室构建并在兔子身上进行评估的HB150和BoHV-1 gG-/tk-菌株，开发了一种减毒标记BoHV-1联合疫苗。本研究旨在使用不同抗原比例进一步评估其在牛中的安全性和保护效力。免疫后，所有接种疫苗的牛直肠温度和精神状态正常，无呼吸道症状。免疫牛的CD4、CD8和CD19细胞显著增加，并诱导了更高的体液和细胞免疫反应，接种疫苗后可促进IL-4、IL-12、TNF-α和IFN-γ等关键细胞因子的表达。1.0×10 CFU的HB150和1.0×10 TCID的BoHV-1 gG-/tk-联合菌株诱导产生的抗体最多，同时在攻毒后显著提高了IgG和细胞免疫。总之，HB150和BoHV-1 gG-/tk-联合菌株在犊牛中临床安全且具有保护作用；1.0×10 CFU的HB150和1.0×10 TCID的BoHV-1 gG-/tk-菌株组合最具前景，因为与其他组合相比，其排毒量低，体液和细胞免疫反应最高。本研究介绍了一种用于养牛业的BoHV-1联合疫苗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/f00fc977b8df/fimmu-15-1367253-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/04fb3e4c29fe/fimmu-15-1367253-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/038b7777e9ef/fimmu-15-1367253-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/33bb1910258a/fimmu-15-1367253-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/bf2cdc4d8851/fimmu-15-1367253-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/f9a17d8a873b/fimmu-15-1367253-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/f00fc977b8df/fimmu-15-1367253-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/04fb3e4c29fe/fimmu-15-1367253-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/038b7777e9ef/fimmu-15-1367253-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/33bb1910258a/fimmu-15-1367253-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/bf2cdc4d8851/fimmu-15-1367253-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/f9a17d8a873b/fimmu-15-1367253-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e2c/11027501/f00fc977b8df/fimmu-15-1367253-g006.jpg

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