Virology Department, Animal Health Research Institute (AHRI), Giza, Egypt.
Department of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Open Vet J. 2024 Aug;14(8):1921-1927. doi: 10.5455/OVJ.2024.v14.i8.20. Epub 2024 Aug 31.
Bovine respiratory disease (BRD) is a complex illness that impacts the respiratory system of domestic cattle, resulting in significant financial losses for the agriculture industry. Inactivated or modified live (MLV) pathogen vaccines are often used as a management tool to prevent and control BRD effectively.
The purpose of this study is to assess the cell-mediated immune response (CMI) induced by two commercially available polyvalent vaccines, namely the MLV (cattle master gold FP) and the inactivated (CATTLEWIN-5K) vaccine.
A total of 20 seronegative heifers against 4 BRD viruses, bovine alphaherpisvirus-1 (BoAHV-1), bovine viral diarrhea virus (BVDV BVDV-1: Pesti virus A; BVDV-2: Pesti virus B), bovine respiratory syncytial virus (BRSV) and bovine parainfluenza virus-3 (BPIV3) were chosen for this study. The heifers were divided into three groups. The first group ( = 6) received no vaccination and was kept as a control. The second and third groups (seven heifers each) were vaccinated twice with either an MLV or inactivated vaccine. The gene expression level of interleukin-6 (IL-6) and interferon-gamma (INF-γ) was measured using real-time quantitative polymerase chain reaction on the 7th, 14th, 21st, 28th, and 60th days post-vaccination. The results were compared with the control group to study the effectiveness of the vaccines.
There was an upregulation in the expression level of IL-6 and INF-γ in both MLV and inactivated vaccinated groups. The level of IL-6 mRNA expression was statistically increased from the 14th and 28th days post-vaccination in MLV and inactivated vaccine groups, respectively. The expression level of INF-γ increased significantly from the 2nd and 4th weeks post-vaccination in the MLV and inactivated vaccine groups, respectively. The mean expression level of IL-6 and INF-γ mRNAs was significantly higher in the MLV vaccine group than in the inactivated vaccine group at each examination time.
Both investigated vaccines are efficient in stimulating CMI, particularly with the MLV vaccine showing a higher preponderance in IL-6 and INF-γ.
牛呼吸道疾病（BRD）是一种影响家畜呼吸系统的复杂疾病，给农业产业造成了重大经济损失。灭活或减毒活（MLV）病原体疫苗常被用作预防和控制 BRD 的管理工具。
本研究旨在评估两种市售多价疫苗，即 MLV（牛主金 FP）和灭活疫苗（CATTLEWIN-5K）引起的细胞介导免疫反应（CMI）。
选择 20 头针对 4 种 BRD 病毒（牛疱疹病毒-1（BoAHV-1）、牛病毒性腹泻病毒（BVDV BVDV-1：Pesti 病毒 A；BVDV-2：Pesti 病毒 B）、牛呼吸道合胞体病毒（BRSV）和牛副流感病毒-3（BPIV3）的血清阴性小母牛进行本研究。小母牛分为三组。第一组（=6）未接种疫苗作为对照。第二组和第三组（每组 7 头）分别用 MLV 或灭活疫苗接种两次。在接种后第 7、14、21、28 和 60 天，使用实时定量聚合酶链反应测量白细胞介素-6（IL-6）和干扰素-γ（INF-γ）的基因表达水平。将结果与对照组进行比较，以研究疫苗的效果。
MLV 和灭活疫苗接种组的 IL-6 和 INF-γ 表达水平均上调。MLV 和灭活疫苗组的 IL-6 mRNA 表达水平分别从接种后第 14 天和第 28 天开始统计学增加。MLV 和灭活疫苗组的 INF-γ 表达水平分别从接种后第 2 周和第 4 周显著增加。在每个检查时间，MLV 疫苗组的 IL-6 和 INF-γ mRNA 的平均表达水平均显著高于灭活疫苗组。
两种研究疫苗均能有效刺激 CMI，尤其是 MLV 疫苗在 IL-6 和 INF-γ 方面表现出更高的优势。
