El-Zahed Mohamed M, Baka Zakaria A, Abou-Dobara Mohamed I, El-Sayed Ahmed K, Aboser Magy M, Hyder Ayman
Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta, 34517, Egypt.
Department of Chemistry, Faculty of Science, Damietta University, New Damietta, 34517, Egypt.
Bioresour Bioprocess. 2021 Jun 3;8(1):44. doi: 10.1186/s40643-021-00400-7.
A novel biosynthesis of dual reduced graphene oxide/silver nanocomposites (rGO/AgNC) using the crude metabolite of Escherichia coli D8 (MF06257) strain and sunlight is introduced in this work. Physicochemical analysis of these rGO/AgNC revealed that they are sheet-like structures having spherically shaped silver nanoparticles (AgNPs) with an average particle size of 8 to 17 nm, and their absorption peak ranged from 350 to 450 nm. The biosynthesized rGO/AgNC were characterized by UV-vis and FT-IR spectra, X-ray diffraction, Zeta potential and transmission electron microscopy. After the injection of these nanocomposites to mice, their uptake by the kidney and liver has been proven by the ultrastructural observation and estimation of the hepatic and renal silver content. These nanocomposites caused a moderate toxicity for both organs. Changes in the liver and kidney functions and histopathological effects had been observed. The rGO/AgNC revealed a remarkable antitumor effect. They showed a dose-dependent cytotoxic effect on Ehrlich ascites carcinoma (EAC) cells in vitro. Treatment of mice bearing EAC tumors intraperitoneally with 10 mg/kg rGO/AgNC showed an antiproliferative effect on EAC cells, reduced ascites volume, and maintained mice survival. The results indicate that this green synergy of silver nanoparticles with reduced graphene oxide may have a promising potential in cancer therapy.
本研究介绍了一种利用大肠杆菌D8（MF06257）菌株的粗代谢产物和阳光，生物合成双还原氧化石墨烯/银纳米复合材料（rGO/AgNC）的新方法。对这些rGO/AgNC的物理化学分析表明，它们是片状结构，具有平均粒径为8至17纳米的球形银纳米颗粒（AgNP），其吸收峰范围为350至450纳米。通过紫外可见光谱和傅里叶变换红外光谱、X射线衍射、Zeta电位和透射电子显微镜对生物合成的rGO/AgNC进行了表征。将这些纳米复合材料注射到小鼠体内后，通过超微结构观察和肝脏及肾脏银含量的测定，证实了它们在肾脏和肝脏中的摄取。这些纳米复合材料对这两个器官都有中等毒性。观察到肝脏和肾脏功能的变化以及组织病理学效应。rGO/AgNC显示出显著的抗肿瘤作用。它们在体外对艾氏腹水癌（EAC）细胞表现出剂量依赖性的细胞毒性作用。用10毫克/千克的rGO/AgNC腹腔注射治疗携带EAC肿瘤的小鼠，对EAC细胞有抗增殖作用，减少腹水体积，并维持小鼠存活。结果表明，银纳米颗粒与还原氧化石墨烯的这种绿色协同作用在癌症治疗中可能具有广阔的前景。
