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NK-溶素抗菌肽生物活性的新见解。

New insight into the biological activity of NK-lysin antimicrobial peptides.

作者信息

Ortega Leonardo, Carrera Crisleri, Muñoz-Flores Carolina, Salazar Santiago, Villegas Milton F, Starck María F, Valenzuela Ariel, Agurto Niza, Montesino Raquel, Astuya Allisson, Parra Natalie, Pérez Ercilia T, Santibáñez Natacha, Romero Alex, Ruíz Pamela, Lamazares Emilio, Reyes Fátima, Sánchez Oliberto, Toledo Jorge R, Acosta Jannel

机构信息

Laboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.

Laboratorio de Piscicultura y Patología Acuática, Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile.

出版信息

Front Immunol. 2024 Apr 9;15:1191966. doi: 10.3389/fimmu.2024.1191966. eCollection 2024.

DOI:10.3389/fimmu.2024.1191966
PMID:38655253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11035819/
Abstract

NK-lysin is a potent antimicrobial peptide (AMP) with antimicrobial activity against bacteria, fungi, viruses, and parasites. NK-lysin is a type of granulysin, a member of the saposin-like proteins family first isolated from a pig's small intestine. In previous work, for the first time, we identified four variants of from Atlantic salmon () using EST sequences. In the present study, we reported and characterized two additional transcripts of from . Besides, we evaluated the tissue distribution of three from and assessed the antimicrobial, hemolytic, and immunomodulatory activities and signaling pathways of three NK-lysin-derived peptides. The synthetic peptides displayed antimicrobial activity against (LF-89) and . These peptides induced the expression of immune genes related to innate and adaptive immune responses and . The immunomodulatory activity of the peptides involves the mitogen-activated protein kinases-mediated signaling pathway, including p38, extracellular signal-regulated kinase 1/2, and/or c-Jun N-terminal kinases. Besides, the peptides modulated the immune response induced by pathogen-associated molecular patterns (PAMPs). Our findings show that NK-lysin could be a highly effective immunostimulant or vaccine adjuvant for use in fish aquaculture.

摘要

自然杀伤细胞溶素是一种具有抗菌活性的强效抗菌肽，对细菌、真菌、病毒和寄生虫均有抗菌作用。自然杀伤细胞溶素是颗粒溶素的一种，属于类鞘脂激活蛋白样蛋白家族成员，最初是从猪的小肠中分离出来的。在之前的工作中，我们首次利用EST序列从大西洋鲑鱼（）中鉴定出四种变体。在本研究中，我们报道并鉴定了来自的另外两种转录本。此外，我们评估了来自的三种的组织分布，并评估了三种自然杀伤细胞溶素衍生肽的抗菌、溶血和免疫调节活性以及信号通路。合成肽对（LF-89）和显示出抗菌活性。这些肽诱导了与先天和适应性免疫反应相关的免疫基因和的表达。肽的免疫调节活性涉及丝裂原活化蛋白激酶介导的信号通路，包括p38、细胞外信号调节激酶1/2和/或c-Jun氨基末端激酶。此外，这些肽调节了由病原体相关分子模式（PAMPs）诱导的免疫反应。我们的研究结果表明，自然杀伤细胞溶素可能是一种用于鱼类养殖的高效免疫刺激剂或疫苗佐剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/b532c2995f80/fimmu-15-1191966-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/0b98e626a36b/fimmu-15-1191966-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/4f2ab5ffb542/fimmu-15-1191966-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/6e0e266ab36a/fimmu-15-1191966-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/b532c2995f80/fimmu-15-1191966-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/8e0c274568c0/fimmu-15-1191966-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/f4caea561234/fimmu-15-1191966-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/5fd025212ccb/fimmu-15-1191966-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/7eee867d2c35/fimmu-15-1191966-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/0b98e626a36b/fimmu-15-1191966-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/f203bf759e44/fimmu-15-1191966-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/6eb9ed54b0b6/fimmu-15-1191966-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c944/11035819/4f2ab5ffb542/fimmu-15-1191966-g009.jpg
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