基于无标记质谱的植物磷蛋白组学中的样品制备和磷酸肽富集。
Sample Preparation and Phosphopeptide Enrichment for Plant Phosphoproteomics via Label-Free Mass Spectrometry.
机构信息
Institute for Bioprocess Science and Engineering (IBSE), Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
Institute of Animal Cell Technology and Systems Biology (IACTSB), Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
出版信息
Methods Mol Biol. 2024;2787:293-303. doi: 10.1007/978-1-0716-3778-4_20.
Phosphopeptide enrichment is the main bottleneck of every phosphorylation study. Therefore, in this chapter, a general workflow tries to overbridge the hurdles of plant sample handling from sample collection to protein extraction, protein solubilization, enzymatic digestion, and enrichment step prior to mass spectrometry. The workflow provides information to perform global proteomics as well as phosphoproteomics enabling the researcher to use the protocol in both fields.
磷酸肽富集是每个磷酸化研究的主要瓶颈。因此，在本章中，我们提出了一个通用的工作流程，旨在克服从样品收集到蛋白质提取、蛋白质溶解、酶解和质谱前富集步骤的植物样品处理的障碍。该工作流程提供了进行全局蛋白质组学和磷酸化蛋白质组学的信息，使研究人员能够在这两个领域中使用该方案。
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