Li Jinna, Silva-Sanchez Cecilia, Zhang Tong, Chen Sixue, Li Haiying
College of Life Sciences, Heilongjiang University Harbin, China.
Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida Gainesville, FL, USA.
Front Plant Sci. 2015 Jun 16;6:430. doi: 10.3389/fpls.2015.00430. eCollection 2015.
Protein phosphorylation has long been recognized as an essential mechanism to regulate many important processes of plant life. However, studies on phosphorylation mediated signaling events in plants are challenged with low stoichiometry and dynamic nature of phosphorylated proteins. Significant advances in mass spectrometry based phosphoproteomics have taken place in recent decade, including phosphoprotein/phosphopeptide enrichment, detection and quantification, and phosphorylation site localization. This review describes a variety of separation and enrichment methods for phosphoproteins and phosphopeptides, the applications of technological innovations in plant phosphoproteomics, and highlights significant achievement of phosphoproteomics in the areas of plant signal transduction, growth and development.
蛋白质磷酸化长期以来一直被认为是调节植物生命许多重要过程的一种基本机制。然而，植物中磷酸化介导的信号事件研究面临着磷酸化蛋白的低化学计量比和动态性质的挑战。近十年来，基于质谱的磷酸化蛋白质组学取得了重大进展，包括磷酸化蛋白/磷酸肽的富集、检测和定量，以及磷酸化位点定位。本文综述了多种磷酸化蛋白和磷酸肽的分离与富集方法、技术创新在植物磷酸化蛋白质组学中的应用，并重点介绍了磷酸化蛋白质组学在植物信号转导、生长和发育领域的重大成就。
