Zhang Xiao-Yu, Yu Ji-Chen, Chen Wen-Tao, Zhou Du-Yan, Yuan Yuan, Liu Hong-Gao, Liang Yan-Li
National-Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest China,Yunnan Agricultural University Kunming 650201,China Yunnan Provincial Key Laboratory of Medicinal Plant Biology,Yunnan Agricultural University Kunming 650201,China College of Agriculture and Biotechnology,Yunnan Agricultural University Kunming 650201,China.
College of Agriculture and Biotechnology,Yunnan Agricultural University Kunming 650201,China.
Zhongguo Zhong Yao Za Zhi. 2024 May;49(9):2422-2433. doi: 10.19540/j.cnki.cjcmm.20240214.101.
The heat shock protein 70 family contains the stress proteins ubiquitous in plants. These proteins are involved in the responses to different abiotic stress conditions and have highly conserved gene sequences. However, little is known about the molecular mechanisms of Fritillaria cirrhosa in response to high-temperature stress. Here, 26 HSP70s, FcHSP70-1 to FcHSP70-26, were identified from the transcriptome data of root, bulb, stem, leaf, and fruit samples of F. cirrhosa. The proteins encoded by FcHSP70s had the lengths ranging from 560 aa to 944 aa, with the molecular weight of 61.64-100.01 kDa and the theoretical isoelectric point between 5.00 and 6.59. The secondary structural elements of HSP70s were mainly random coils and α-helixes. Subcellular localization prediction revealed that FcHSP70s were distributed in mitochondria, chloroplasts, nuclei, endoplasmic reticulum, and cytoplasm. The phylogenetic tree showed that 7 members of the HSP70 family belonged to the Dnak subfamily and 19 members belonged to the HSP110/SSE subfamily. In addition, the qRT-PCR results showed that the expression of FcHSP70-5, FcHSP70-8, FcHSP70-17, FcHSP70-18, and FcHSP70-23 in F. cirrhosa was significantly up-regulated at 35 ℃, which indicated that these genes might play a role in the response to high temperature stress. In addition, compared with other tissues, stems and leaves were sensitive to high temperature stress, with the expression of 18 genes up-regulated by 18.18 and 8.03 folds on average, respectively. These findings provide valuable information about the molecular mechanism of HSP70s of F. cirrhosa in response to high temperature stress.
热休克蛋白70家族包含植物中普遍存在的应激蛋白。这些蛋白质参与对不同非生物胁迫条件的响应，并且具有高度保守的基因序列。然而，关于川贝母响应高温胁迫的分子机制知之甚少。在此，从川贝母根、鳞茎、茎、叶和果实样本的转录组数据中鉴定出26个HSP70，即FcHSP70-1至FcHSP70-26。FcHSP70编码的蛋白质长度范围为560个氨基酸至944个氨基酸，分子量为61.64-100.01 kDa，理论等电点在5.00至6.59之间。HSP70的二级结构元件主要是无规卷曲和α-螺旋。亚细胞定位预测显示FcHSP70分布在线粒体、叶绿体、细胞核、内质网和细胞质中。系统发育树表明，HSP70家族的7个成员属于Dnak亚家族，19个成员属于HSP110/SSE亚家族。此外，qRT-PCR结果表明，川贝母中FcHSP70-5、FcHSP70-8、FcHSP70-17、FcHSP70-18和FcHSP70-23在35℃时表达显著上调，这表明这些基因可能在响应高温胁迫中发挥作用。此外，与其他组织相比，茎和叶对高温胁迫敏感，18个基因的表达分别平均上调了18.18倍和8.03倍。这些发现为川贝母HSP70响应高温胁迫的分子机制提供了有价值的信息。
