Geukens Tatjana, De Schepper Maxim, Van Den Bogaert Wouter, Van Baelen Karen, Maetens Marion, Pabba Anirudh, Mahdami Amena, Leduc Sophia, Isnaldi Edoardo, Nguyen Ha-Linh, Bachir Imane, Hajipirloo Maysam, Zels Gitte, Van Cauwenberge Josephine, Borremans Kristien, Vandecaveye Vincent, Weynand Birgit, Vermeulen Peter, Leucci Eleonora, Baietti Maria Francesca, Sflomos George, Battista Laura, Brisken Cathrin, Derksen Patrick W B, Koorman Thijs, Visser Daan, Scheele Colinda L G J, Thommen Daniela S, Hatse Sigrid, Fendt Sarah-Maria, Vanderheyden Evy, Van Brussel Thomas, Schepers Rogier, Boeckx Bram, Lambrechts Diether, Marano Giuseppe, Biganzoli Elia, Smeets Ann, Nevelsteen Ines, Punie Kevin, Neven Patrick, Wildiers Hans, Richard François, Floris Giuseppe, Desmedt Christine
Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
Department of General Medical Oncology, University Hospitals Leuven, Leuven, Belgium.
NPJ Breast Cancer. 2024 Apr 24;10(1):31. doi: 10.1038/s41523-024-00637-3.
Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast cancer post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median of 31 (range: 5-90) metastases and 5-8 liquids per patient from its first 20 patients. In a dedicated experiment, we show the mild impact of increasing time after death on RNA quality, transcriptional profiles and immunohistochemical staining in tumor tissue samples. We show that this impact can be counteracted by organ cooling. We successfully generated ex vivo models from tissue and liquid biopsies from distinct histological subtypes of breast cancer. We anticipate these and future findings of UPTIDER to elucidate mechanisms of disease progression and treatment resistance and to provide tools for the exploration of precision medicine strategies in the metastatic setting.
转移性癌症的研究一直受到样本获取有限的阻碍。在此，我们介绍乳腺癌尸检组织捐赠项目UPTIDER，并展示该项目如何使前20名患者平均每人能够获取31个（范围：5 - 90个）转移灶样本以及5 - 8份液体样本。在一项专门实验中，我们表明死亡后时间延长对肿瘤组织样本中的RNA质量、转录谱和免疫组化染色的影响较小。我们还表明这种影响可通过器官冷却来抵消。我们成功地从不同组织学亚型的乳腺癌组织和液体活检样本中建立了体外模型。我们预计UPTIDER的这些及未来发现将阐明疾病进展和治疗耐药的机制，并为探索转移性疾病环境下的精准医学策略提供工具。
