Schøller J, Lernmark A
Immunogenetics. 1985;22(6):601-8. doi: 10.1007/BF00430308.
A human HLA-DQ beta-chain cDNA was used as a probe to identify and isolate a rat major histocompatibility antigen beta-chain gene from a genomic library constructed in the vector lambda Charon 28 using Wistar rat DNA (RT1u). The isolated exon of the rat gene (RT1.B beta 2) encoding a beta-chain second domain was found to share 93% nucleotide homology with a mouse A beta 2 exon. Although the genomic organization of this gene is consistent with the hypothesis that it represents a pseudogene, the remarkable preservation of a specific sequence favors the view that this class II antigen beta-chain gene has retained its coding function.
使用人类HLA-DQβ链cDNA作为探针，从用Wistar大鼠DNA（RT1u）构建于载体λ噬菌体Charon 28中的基因组文库中鉴定并分离出大鼠主要组织相容性抗原β链基因。发现大鼠基因（RT1.Bβ2）编码β链第二结构域的分离外显子与小鼠Aβ2外显子具有93%的核苷酸同源性。尽管该基因的基因组组织与它代表假基因的假设一致，但特定序列的显著保留支持了这种II类抗原β链基因保留了其编码功能的观点。
