Eccles S J, McMaster W R
Immunogenetics. 1985;22(6):653-63. doi: 10.1007/BF00430314.
The rat major histocompatibility complex (RT1) encodes twin sets of class II molecules, each consisting of two polypeptide chains referred to as A alpha and A beta, and E alpha and E beta. A gene encoding the RT1.A beta chain was isolated from a rat genomic library using an HLA-DQ beta chain cDNA as a probe. The nucleotide sequence of the coding regions of this gene was determined. Comparison of this sequence with those of the corresponding genes of mouse (H-2A beta) and human (HLA-DQ beta) revealed that this gene has been highly conserved during evolution, and that some parts of the molecule are more conserved than others. Analysis of the nucleotide sequence encoding the two external domains suggests that the membrane proximal domain has been subject to conservative selection, whereas replacement substitutions have been selected positively at certain residues within the amino terminal domain. The overall organization of the RT1.A beta gene is similar to that of the H-2A beta gene.
大鼠主要组织相容性复合体（RT1）编码两组Ⅱ类分子，每组由两条多肽链组成，分别称为Aα和Aβ以及Eα和Eβ。以HLA - DQβ链cDNA为探针，从大鼠基因组文库中分离出一个编码RT1.Aβ链的基因。测定了该基因编码区的核苷酸序列。将该序列与小鼠（H - 2Aβ）和人类（HLA - DQβ）相应基因的序列进行比较，发现该基因在进化过程中高度保守，且分子的某些部分比其他部分更保守。对编码两个外部结构域的核苷酸序列分析表明，膜近端结构域受到保守选择，而在氨基末端结构域的某些残基处，替换性替代则受到正向选择。RT1.Aβ基因的整体结构与H - 2Aβ基因相似。
