Zhao Qinsong, Dong Jingjing, Li Shibiao, Lei Wenxin, Liu Ake
Department of Life Sciences, Changzhi University, Changzhi, China.
Front Plant Sci. 2024 Apr 11;15:1393905. doi: 10.3389/fpls.2024.1393905. eCollection 2024.
Due to its high efficacy as a wide-spectrum disinfectant and its potential for the degradation of pollutants and pesticides, ozone has broad application prospects in agricultural production. In this study, micro/nano bubble technology was applied to achieve a saturation state of bubble nutrient solution, including micro-nano oxygen (O group) and micro-nano ozone (O group) bubble nutrient solutions. The effects of these solutions on lettuce physiological indices as well as changes in the microbial community within the rhizosphere substrate were studied. The application of micro/nano (O and O) bubble nutrient solutions to substrate-cultured lettuce plants increased the amount of dissolved oxygen in the nutrient solution, increased the lettuce yield, and elevated the net photosynthetic rate, conductance of HO and intercellular carbon dioxide concentration of lettuce plants. Diversity analysis of the rhizosphere microbial community revealed that both the abundance and diversity of bacterial and fungal communities in the substrate increased after plant cultivation and decreased following treatment with micro/nanobubble nutrient solutions. RDA results showed that the microbial community in the S group was positively associated with EC, that in the CK and O groups exhibited a positive correlation with SC, and that in the O group displayed a positive correlation with CAT and POD. Overall, the implementation of micro/nanobubble generation technology in soilless substrates can effectively increase the lettuce growth and yield, and O had a more pronounced effect on lettuce yield and quality and the microbial community structure in the substrate than O. Our study would provide a reference and theoretical basis for developing sustainable and green technology for promoting lettuce production and can be a promising alternative to conventional methods for improving crop yields.
由于臭氧作为一种广谱消毒剂具有高效性，且具有降解污染物和农药的潜力，因此在农业生产中具有广阔的应用前景。在本研究中，应用微/纳米气泡技术使气泡营养液达到饱和状态，包括微纳米氧气（O组）和微纳米臭氧（O组）气泡营养液。研究了这些溶液对生菜生理指标的影响以及根际基质中微生物群落的变化。将微/纳米（O和O）气泡营养液应用于基质栽培的生菜植株，增加了营养液中的溶解氧含量，提高了生菜产量，提升了生菜植株的净光合速率、HO导度和胞间二氧化碳浓度。根际微生物群落多样性分析表明，种植后基质中细菌和真菌群落的丰度和多样性均增加，而经微/纳米气泡营养液处理后则降低。冗余分析结果表明，S组中的微生物群落与电导率呈正相关，CK组和O组中的微生物群落与可溶性碳呈正相关，O组中的微生物群落与过氧化氢酶和过氧化物酶呈正相关。总体而言，在无土基质中实施微/纳米气泡生成技术可有效促进生菜生长并提高产量，且O对生菜产量、品质及基质中微生物群落结构的影响比O更为显著。本研究将为开发促进生菜生产的可持续绿色技术提供参考和理论依据，并且有望成为提高作物产量的传统方法的替代方案。
