Wang Liyang, Wu Hongxuan, He Wei, Lai Guihong, Li Junxi, Liu Siling, Zhou Qiang
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
Insects. 2024 Mar 26;15(4):228. doi: 10.3390/insects15040228.
A comprehensive and precise evaluation of Arthropoda diversity in agricultural landscapes can enhance biological pest control strategies. We used Malaise traps and sweep nets to collect insects from three double-cropping paddy fields. DNA was extracted from the ethanol preservative of the Malaise traps and from tissue samples of selected parasitoid wasps. This was followed by amplification using DNA barcoding primers to prepare high-throughput sequencing libraries. We annotated a total of 4956 operational taxonomic units (OTUs), encompassing 174 genera and 32 families of parasitoid wasps. The ethanol filter method efficiently captured a wide range of information. However, the method has low resolution and may result in a reduced estimate of species abundance. Additional insect species were also identified in the parasitoid samples. This suggests that high throughput sequencing from adult parasitoid wasps can also detect host species, enabling a better understanding of host species and providing insights into food webs.
对农业景观中节肢动物多样性进行全面而精确的评估可以加强生物害虫防治策略。我们使用马氏网和扫网从三块双季稻田中采集昆虫。从马氏网的乙醇保存液和选定寄生蜂的组织样本中提取DNA。随后使用DNA条形码引物进行扩增，以制备高通量测序文库。我们总共注释了4956个操作分类单元（OTU），涵盖174个属和32个寄生蜂科。乙醇过滤法有效地捕获了广泛的信息。然而，该方法分辨率较低，可能会导致物种丰度估计值降低。在寄生蜂样本中还鉴定出了其他昆虫物种。这表明从成年寄生蜂进行高通量测序也可以检测宿主物种，从而更好地了解宿主物种并深入了解食物网。
