• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

海洋真菌来源的天然化合物 4-羟基苯乙酸通过诱导自噬在斑马鱼中发挥抗血栓作用。

Marine-Fungus-Derived Natural Compound 4-Hydroxyphenylacetic Acid Induces Autophagy to Exert Antithrombotic Effects in Zebrafish.

机构信息

Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China.

Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs, Key Laboratory of Agro-Products Processing Technology of Shandong Province, Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, 23788 Gongye North Road, Jinan 250100, China.

出版信息

Mar Drugs. 2024 Mar 27;22(4):148. doi: 10.3390/md22040148.

DOI:10.3390/md22040148
PMID:38667765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11051058/
Abstract

Marine natural products are important sources of novel drugs. In this study, we isolated 4-hydroxyphenylacetic acid (HPA) from the marine-derived fungus Y39-2. The antithrombotic activity and mechanism of HPA were reported for the first time. Using a zebrafish model, we found that HPA had a strong antithrombotic activity because it can significantly increase cardiac erythrocytes, blood flow velocity, and heart rate, reduce caudal thrombus, and reverse the inflammatory response caused by Arachidonic Acid (AA). Further transcriptome analysis and qRT-PCR validation demonstrated that HPA may regulate autophagy by inhibiting the PI3K/AKT/mTOR signaling pathway to exert antithrombotic effects.

摘要

海洋天然产物是新型药物的重要来源。在这项研究中，我们从海洋来源的真菌 Y39-2 中分离出 4-羟基苯乙酸（HPA）。HPA 的抗血栓活性及其机制尚属首次报道。通过斑马鱼模型，我们发现 HPA 具有很强的抗血栓活性，因为它可以显著增加心脏红细胞、血流速度和心率，减少尾部血栓，并逆转花生四烯酸（AA）引起的炎症反应。进一步的转录组分析和 qRT-PCR 验证表明，HPA 可能通过抑制 PI3K/AKT/mTOR 信号通路来调节自噬，从而发挥抗血栓作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/8dbdc082e050/marinedrugs-22-00148-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/498ba2a0554d/marinedrugs-22-00148-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/a1e098ac91f5/marinedrugs-22-00148-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/aee7f31c92e8/marinedrugs-22-00148-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/c3ddd75fc362/marinedrugs-22-00148-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/355573836336/marinedrugs-22-00148-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/00947de21e5f/marinedrugs-22-00148-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/6ec58833f7c8/marinedrugs-22-00148-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/bc3e76c374a5/marinedrugs-22-00148-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/635dcc0d7fcf/marinedrugs-22-00148-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/8dbdc082e050/marinedrugs-22-00148-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/498ba2a0554d/marinedrugs-22-00148-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/a1e098ac91f5/marinedrugs-22-00148-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/aee7f31c92e8/marinedrugs-22-00148-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/c3ddd75fc362/marinedrugs-22-00148-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/355573836336/marinedrugs-22-00148-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/00947de21e5f/marinedrugs-22-00148-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/6ec58833f7c8/marinedrugs-22-00148-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/bc3e76c374a5/marinedrugs-22-00148-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/635dcc0d7fcf/marinedrugs-22-00148-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce2c/11051058/8dbdc082e050/marinedrugs-22-00148-g010.jpg

相似文献

1
Marine-Fungus-Derived Natural Compound 4-Hydroxyphenylacetic Acid Induces Autophagy to Exert Antithrombotic Effects in Zebrafish.海洋真菌来源的天然化合物 4-羟基苯乙酸通过诱导自噬在斑马鱼中发挥抗血栓作用。
Mar Drugs. 2024 Mar 27;22(4):148. doi: 10.3390/md22040148.
2
Antiplatelet and Antithrombotic Effects of Isaridin E Isolated from the Marine-Derived Fungus via Downregulating the PI3K/Akt Signaling Pathway.通过下调 PI3K/Akt 信号通路从海洋来源真菌中分离得到的异土木香内酯的抗血小板和抗血栓形成作用。
Mar Drugs. 2021 Dec 24;20(1):23. doi: 10.3390/md20010023.
3
Discovery and identification of antithrombotic chemical markers in Gardenia Fructus by herbal metabolomics and zebrafish model.基于草药代谢组学和斑马鱼模型发现并鉴定栀子中的抗血栓化学标志物。
J Ethnopharmacol. 2020 May 10;253:112679. doi: 10.1016/j.jep.2020.112679. Epub 2020 Feb 23.
4
Therapeutic effects and molecular mechanisms of natural products in thrombosis.天然产物在血栓形成中的治疗作用及分子机制。
Phytother Res. 2024 May;38(5):2128-2153. doi: 10.1002/ptr.8151. Epub 2024 Feb 23.
5
Petromurin C Induces Protective Autophagy and Apoptosis in FLT3-ITD-Positive AML: Synergy with Gilteritinib.Petromurin C 诱导 FLT3-ITD 阳性 AML 中的保护性自噬和细胞凋亡：与吉特替尼协同作用。
Mar Drugs. 2020 Jan 16;18(1):57. doi: 10.3390/md18010057.
6
Recent advancement of novel marine fungi derived secondary metabolite fibrinolytic compound FGFC in biomedical applications: a review.新型海洋真菌来源的次级代谢产物纤溶化合物 FGFC 在生物医药应用中的研究进展：综述。
Front Cell Infect Microbiol. 2024 Sep 18;14:1422648. doi: 10.3389/fcimb.2024.1422648. eCollection 2024.
7
Natural products targeting autophagy via the PI3K/Akt/mTOR pathway as anticancer agents.通过 PI3K/Akt/mTOR 通路靶向自噬的天然产物作为抗癌剂。
Anticancer Agents Med Chem. 2013 Sep;13(7):1048-56. doi: 10.2174/18715206113139990130.
8
Marine cyclotripeptide X-13 promotes angiogenesis in zebrafish and human endothelial cells via PI3K/Akt/eNOS signaling pathways.海洋环三肽 X-13 通过 PI3K/Akt/eNOS 信号通路促进斑马鱼和人内皮细胞的血管生成。
Mar Drugs. 2012 Jun;10(6):1307-1320. doi: 10.3390/md10061307. Epub 2012 Jun 7.
9
Alleviation of endothelial dysfunction of Pheretima guillemi (Michaelsen)-derived protein DPf3 in ponatinib-induced thrombotic zebrafish and mechanisms explored through ox-LDL-induced HUVECs and TMT-based proteomics.白珍珠蛭素 DPf3 缓解帕替尼诱导血栓斑马鱼的血管内皮功能障碍及 ox-LDL 诱导 HUVECs 和 TMT 蛋白质组学探讨的机制。
J Ethnopharmacol. 2024 Apr 6;323:117669. doi: 10.1016/j.jep.2023.117669. Epub 2023 Dec 28.
10
A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs.一种用于评估抗血栓药物的斑马鱼血栓形成模型。
Zebrafish. 2016 Aug;13(4):335-44. doi: 10.1089/zeb.2016.1263.

引用本文的文献

1
Antithrombotic Effect of L. Extract and Its Fractions via Regulating TLRs and the Downstream MAPKs and PI3K/AKT Signaling Pathways in Zebrafish.L.提取物及其馏分通过调节斑马鱼中的Toll样受体（TLRs）以及下游的丝裂原活化蛋白激酶（MAPKs）和磷脂酰肌醇-3激酶/蛋白激酶B（PI3K/AKT）信号通路产生的抗血栓形成作用
Int J Mol Sci. 2025 Feb 27;26(5):2118. doi: 10.3390/ijms26052118.
2
Recent Advances of the Zebrafish Model in the Discovery of Marine Bioactive Molecules.斑马鱼模型在海洋生物活性分子发现中的最新进展
Mar Drugs. 2024 Nov 30;22(12):540. doi: 10.3390/md22120540.
3
Brevianamide F Exerts Antithrombotic Effects by Modulating the MAPK Signaling Pathway and Coagulation Cascade.

本文引用的文献

1
Research progress of NF-κB signaling pathway and thrombosis.NF-κB 信号通路与血栓的研究进展。
Front Immunol. 2023 Sep 29;14:1257988. doi: 10.3389/fimmu.2023.1257988. eCollection 2023.
2
Gut-derived 4-hydroxyphenylacetic acid attenuates sepsis-induced acute kidney injury by upregulating ARC to inhibit necroptosis.肠源 4- 轻基苯乙酸通过上调 ARC 抑制坏死性凋亡来减轻脓毒症诱导的急性肾损伤。
Biochim Biophys Acta Mol Basis Dis. 2024 Jan;1870(1):166876. doi: 10.1016/j.bbadis.2023.166876. Epub 2023 Sep 13.
3
The fungal metabolite 4-hydroxyphenylacetic acid from Neofusicoccum parvum modulates defence responses in grapevine.
布瑞维亚胺 F 通过调节 MAPK 信号通路和凝血级联发挥抗血栓作用。
Mar Drugs. 2024 Sep 26;22(10):439. doi: 10.3390/md22100439.
来自葡萄座腔菌的真菌代谢产物4-羟基苯乙酸调节葡萄的防御反应。
Plant Cell Environ. 2023 Nov;46(11):3575-3591. doi: 10.1111/pce.14670. Epub 2023 Jul 11.
4
Evaluation of Lipid Extracts from the Marine Fungi and as a Source of Anti-Inflammatory, Antioxidant and Antibacterial Compounds.海洋真菌 和 的脂质提取物作为抗炎、抗氧化和抗菌化合物的来源评估。
Mar Drugs. 2023 Mar 23;21(4):199. doi: 10.3390/md21040199.
5
Wuliangye Baijiu but not ethanol reduces cardiovascular disease risks in a zebrafish thrombosis model.在斑马鱼血栓形成模型中，五粮液白酒而非乙醇可降低心血管疾病风险。
NPJ Sci Food. 2022 Dec 5;6(1):55. doi: 10.1038/s41538-022-00170-2.
6
Marine Natural Products in Clinical Use.临床应用中的海洋天然产物。
Mar Drugs. 2022 Aug 18;20(8):528. doi: 10.3390/md20080528.
7
Exploring Peptaibol's Profile, Antifungal, and Antitumor Activity of Emericellipsin A of Species from Soda and Saline Soils.探讨来自苏打和盐渍土壤物种的 Emericellipsin A 的 Peptaibol 特性、抗真菌和抗肿瘤活性。
Molecules. 2022 Mar 7;27(5):1736. doi: 10.3390/molecules27051736.
8
Marine Microbial Fibrinolytic Enzymes: An Overview of Source, Production, Biochemical Properties and Thrombolytic Activity.海洋微生物溶栓酶：来源、生产、生化特性和溶栓活性概述。
Mar Drugs. 2022 Jan 2;20(1):46. doi: 10.3390/md20010046.
9
The genus Simplicillium and Emericellopsis: A review of phytochemistry and pharmacology.《Simplicillium 属和 Emericellopsis 属：植物化学和药理学综述》。
Biotechnol Appl Biochem. 2022 Oct;69(5):2229-2239. doi: 10.1002/bab.2281. Epub 2021 Dec 1.
10
Deaths: Leading Causes for 2019.死亡：2019 年的主要死因。
Natl Vital Stat Rep. 2021 Jul;70(9):1-114.