Sánchez-Torres Paloma, González-Candelas Luis, Ballester Ana Rosa
Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Moncada, 46113 Valencia, Spain.
Food Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Catedrático Agustín Escardino Benlloch 7, Paterna, 46980 Valencia, Spain.
J Fungi (Basel). 2024 Mar 22;10(4):235. doi: 10.3390/jof10040235.
Green mold caused by (Pers.:Fr.) Sacc is the most prevalent postharvest rot concerning citrus fruits. Using the subtractive suppression hybridization (SSH) technique, different genes have been identified that could be involved in virulence during citrus infection in the early stages, a crucial moment that determines whether the infection progresses or not. To this end, a comparison of two strains with high and low virulence has been carried out. We conducted a study on the gene expression profile of the most relevant genes. The results indicate the importance of transcription and regulation processes as well as enzymes involved in the degradation of the plant cell wall. The most represented expressed sequence tag (EST) was identified as PDIP_11000, associated with the FluG domain, which is putatively involved in the activation of conidiation. It is also worth noting that PDIP_02280 encodes a pectin methyl esterase, a cell wall remodeling protein with a high expression level in the most virulent fungal strains, which is notably induced during citrus infection. Furthermore, within the group with the greatest representation and showing significant induction in the early stages of infection, regulatory proteins (PDIP_68700, PDIP_76160) and a chaperone (PDIP_38040) stand out. To a lesser extent, but not less relevant, it is worth distinguishing different regulatory proteins and transcription factors, such as PDIP_00580, PDIP_49640 and PDIP_78930.
由（Pers.:Fr.）萨卡氏菌引起的绿霉病是柑橘类水果采后最普遍的腐烂病害。利用抑制性消减杂交（SSH）技术，已鉴定出不同的基因，这些基因可能在柑橘感染早期的致病过程中发挥作用，这是决定感染是否会发展的关键阶段。为此，对两株毒力高低不同的菌株进行了比较。我们对最相关基因的基因表达谱进行了研究。结果表明转录和调控过程以及参与植物细胞壁降解的酶的重要性。最具代表性的表达序列标签（EST）被鉴定为PDIP_11000，与FluG结构域相关，推测其参与分生孢子形成的激活。还值得注意的是，PDIP_02280编码一种果胶甲酯酶，这是一种在毒性最强的真菌菌株中高表达的细胞壁重塑蛋白，在柑橘感染期间显著诱导表达。此外，在感染早期代表性最强且显示出显著诱导的基因组中，调控蛋白（PDIP_68700、PDIP_76160）和一种伴侣蛋白（PDIP_38040）尤为突出。在较小程度上但并非不相关的是，值得区分不同的调控蛋白和转录因子，如PDIP_00580、PDIP_49640和PDIP_78930。
