Environmental and Municipal Engineering Department, Xi' an University of Architecture and Technology, Xi'an, Shaanxi, China.
Environmental and Municipal Engineering Department, Xi' an University of Architecture and Technology, Xi'an, Shaanxi, China; Key Laboratory of Northwest Water Resources, Environment and Ecology, Ministry of Education, China; Key Laboratory of Environmental Engineering, Shaanxi, China.
J Hazard Mater. 2024 Jun 5;471:134255. doi: 10.1016/j.jhazmat.2024.134255. Epub 2024 Apr 9.
In recent years, large quantities of pharmaceuticals and personal care products (PPCPs) have been discharged into sewers, while the mechanisms of PPCPs enrichment in sewer sediments have rarely been revealed. In this study, three PPCPs (tetracycline, sulfamethoxazole, and triclocarban) were added consecutively over a 90-day experimental period to reveal the mechanisms of PPCPs enrichment and the transmission of resistance genes in sewer sediments. The results showed that tetracycline (TC) and triclocarban (TCC) have higher adsorption concentration in sediments compared to sulfamethoxazole (SMX). The absolute abundance of Tets and suls genes increased in sediments under PPCPs pressure. The increase in secretion of extracellular polymeric substances (EPS) and the loosening of the structure exposed a large number of hydrophobic functional groups, which promoted the adsorption of PPCPs. The absolute abundance of antibiotic resistance genes (ARGs), EPS and the content of PPCPs in sediments exhibited significant correlations. The enrichment of PPCPs in sediments was attributed to the accumulation of EPS, which led to the proliferation of ARGs. These findings contributed to further understanding of the fate of PPCPs in sewer sediments and opened a new perspective for consideration of controlling the proliferation of resistance genes.
近年来，大量的药品和个人护理产品（PPCPs）被排放到污水中，而 PPCPs 在污水沉积物中富集的机制却很少被揭示。在这项研究中，连续添加了三种 PPCPs（四环素、磺胺甲恶唑和三氯生），历时 90 天，以揭示 PPCPs 富集和抗性基因在污水沉积物中传播的机制。结果表明，与磺胺甲恶唑（SMX）相比，四环素（TC）和三氯生（TCC）在沉积物中的吸附浓度更高。在 PPCPs 压力下，Tets 和 suls 基因的绝对丰度在沉积物中增加。细胞外聚合物（EPS）的分泌增加和结构的松动暴露了大量的疏水性官能团，这促进了 PPCPs 的吸附。抗生素抗性基因（ARGs）、EPS 和沉积物中 PPCPs 的绝对丰度之间存在显著相关性。PPCPs 在沉积物中的富集归因于 EPS 的积累，这导致了 ARGs 的增殖。这些发现有助于进一步了解 PPCPs 在污水沉积物中的归宿，并为控制抗性基因的增殖提供了新的视角。
