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全基因组重测序与转录组测序相结合揭示蛋壳高光泽度的候选基因。

Integration of Whole-Genome Resequencing and Transcriptome Sequencing Reveals Candidate Genes in High Glossiness of Eggshell.

作者信息

Song Xiang, Li Shuo, He Shixiong, Zheng Hongxiang, Li Ruijie, Liu Long, Geng Tuoyu, Zhao Minmeng, Gong Daoqing

机构信息

College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

Jiangsu Beinongda Agriculture and Animal Husbandry Technology Co., Ltd., Taizhou 225300, China.

出版信息

Animals (Basel). 2024 Apr 9;14(8):1141. doi: 10.3390/ani14081141.

DOI:10.3390/ani14081141
PMID:38672292
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11047648/
Abstract

Eggshell gloss is an important characteristic for the manifestation of eggshell appearance. However, no study has yet identified potential candidate genes for eggshell gloss between high-gloss (HG) and low-gloss (LG) chickens. The aim of this study was to perform a preliminary investigation into the formation mechanism of eggshell gloss and to identify potential genes. The eggshell gloss of 300-day-old Rhode Island Red hens was measured from three aspects. Uterine tissues of the selected HG and LG ( = 5) hens were collected for RNA-seq. Blood samples were also collected for whole-genome resequencing (WGRS). RNA-seq analysis showed that 150 differentially expressed genes (DEGs) were identified in the uterine tissues of HG and LG hens. These DEGs were mainly enriched in the calcium signaling pathway and the neuroactive ligand-receptor interaction pathway. Importantly, these two pathways were also significantly enriched in the WGRS analysis results. Further joint analysis of WGRS and RNA-seq data revealed that 5-hydroxytryptamine receptor 1F (), zinc finger protein 536 (), NEDD8 ubiquitin-like modifier (), nerve growth factor () and calmodulin 1 () are potential candidate genes for eggshell gloss. In summary, our research provides a reference for the study of eggshell gloss and lays a foundation for improving egg glossiness in layer breeding.

摘要

蛋壳光泽是蛋壳外观表现的一个重要特征。然而，尚未有研究确定高光泽（HG）和低光泽（LG）鸡之间蛋壳光泽的潜在候选基因。本研究的目的是对蛋壳光泽的形成机制进行初步研究并鉴定潜在基因。从三个方面测量了300日龄罗德岛红母鸡的蛋壳光泽。采集了选定的HG和LG（ = 5）母鸡的子宫组织用于RNA测序。还采集了血液样本用于全基因组重测序（WGRS）。RNA测序分析表明，在HG和LG母鸡的子宫组织中鉴定出150个差异表达基因（DEG）。这些DEG主要富集在钙信号通路和神经活性配体-受体相互作用通路中。重要的是，这两条通路在WGRS分析结果中也显著富集。对WGRS和RNA测序数据的进一步联合分析表明，5-羟色胺受体1F（）、锌指蛋白536（）、NEDD8类泛素修饰物（）、神经生长因子（）和钙调蛋白1（）是蛋壳光泽的潜在候选基因。总之，我们的研究为蛋壳光泽的研究提供了参考，并为提高蛋鸡育种中的蛋光泽度奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/1f1cc62ecfb4/animals-14-01141-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/5ca89333ac09/animals-14-01141-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/c6f7feed23b4/animals-14-01141-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/a21e9d2c93cd/animals-14-01141-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/e3d706bc1cb4/animals-14-01141-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/1f1cc62ecfb4/animals-14-01141-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/5ca89333ac09/animals-14-01141-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/c6f7feed23b4/animals-14-01141-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/a21e9d2c93cd/animals-14-01141-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/e3d706bc1cb4/animals-14-01141-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3855/11047648/1f1cc62ecfb4/animals-14-01141-g005.jpg

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