Ding Jiqiang, Ying Fan, Li Qinghe, Zhang Gaomeng, Zhang Jin, Liu Ranran, Zheng Maiqing, Wen Jie, Zhao Guiping
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
MiLe Xinguang Agricultural and Animal Industrials Corporation, MiLe, China.
J Anim Sci Biotechnol. 2022 Aug 9;13(1):96. doi: 10.1186/s40104-022-00744-w.
Egg production is economically important in the meat-type chicken industry. To better understand the molecular genetic mechanism of egg production in meat-type chicken, genetic parameter estimation, genome-wide association analyses combined with meta-analyses, Bayesian analyses, and selective sweep analyses were performed to screen single nucleotide polymorphisms (SNPs) and other genetic loci that were significantly associated with egg number traits in 11,279 chickens from seven material lines.
Yellow-feathered meat-type chickens laid 115 eggs at 43 weeks of age and white-feathered chickens laid 143 eggs at 60 weeks of age, with heritability ranging from 0.034-0.258. Based on meta-analyses and selective sweep analyses, one region (10.81-13.05 Mb) on chromosome Z was associated with egg number in all lines. Further analyses using the W2 line was also associated with the same region, and 29 SNPs were identified that significantly affected estimation of breeding value of egg numbers. The 29 SNPs were identified as having a significant effect on the egg number EBV in 3194 birds in line W2. There are 36 genes in the region, with glial cell derived neurotrophic factor, DAB adaptor protein 2, protein kinase AMP-activated catalytic subunit alpha 1, NAD kinase 2, mitochondrial, WD repeat domain 70, leukemia inhibitory factor receptor alpha, complement C6, and complement C7 identified as being potentially affecting to egg number. In addition, three SNPs (rs318154184, rs13769886, and rs313325646) associated with egg number were located on or near the prolactin receptor gene.
Our study used genomic information from different chicken lines and populations to identify a genomic region (spanning 2.24 Mb) associated with egg number. Nine genes and 29 SNPs were identified as the most likely candidate genes and variations for egg production. These results contribute to the identification of candidate genes and variants for egg traits in poultry.
产蛋在肉用型鸡产业中具有重要的经济意义。为了更好地理解肉用型鸡产蛋的分子遗传机制，我们进行了遗传参数估计、全基因组关联分析，并结合荟萃分析、贝叶斯分析和选择清除分析，以筛选来自七个品系的11279只鸡中与产蛋数性状显著相关的单核苷酸多态性（SNP）和其他遗传位点。
黄羽肉用型鸡在43周龄时产蛋115枚，白羽鸡在60周龄时产蛋143枚，遗传力范围为0.034 - 0.258。基于荟萃分析和选择清除分析，Z染色体上的一个区域（10.81 - 13.05 Mb）与所有品系的产蛋数相关。使用W2品系进行的进一步分析也与同一区域相关，并鉴定出29个SNP，它们显著影响产蛋数育种值的估计。在W2品系的3194只鸡中，这29个SNP被确定对产蛋数估计育种值有显著影响。该区域有36个基因，其中胶质细胞源性神经营养因子、DAB衔接蛋白2、蛋白激酶AMP激活的催化亚基α1、NAD激酶2（线粒体）、WD重复结构域70、白血病抑制因子受体α、补体C6和补体C7被确定可能影响产蛋数。此外，三个与产蛋数相关的SNP（rs318154184、rs13769886和rs313325646）位于催乳素受体基因上或其附近。
我们的研究利用来自不同鸡品系和群体的基因组信息，鉴定出一个与产蛋数相关的基因组区域（跨度为2.24 Mb）。九个基因和29个SNP被确定为最有可能的产蛋候选基因和变异。这些结果有助于鉴定家禽产蛋性状的候选基因和变异。
