Gao Yuhan, Li Xiaomin, Xu Hui, Sun Huijuan, Zhang Junli, Wu Xiaoping, Fu Junsheng
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Chemical and Biomolecular Engineering Department, College of Design and Engineering, National University of Singapore, Singapore 117585, Singapore.
Foods. 2024 Apr 13;13(8):1190. doi: 10.3390/foods13081190.
is a rare fungus growing on mulberry trees that has immense medicinal value. This study aimed to optimize the liquid-fermentation-media formulation and culture conditions for large-scale culturing of by performing one-way testing and response surface methodology. The antioxidant and anticancer activities of the extracellular polysaccharides from were also analyzed. The optimal formulation and growth conditions for were as follows: glucose, 30.2 ± 0.37 g/L; yeast extract, 14.60 ± 0.05 g/L; dandelion powder, 1.24 ± 0.01 g/L; shaker speed, 150 r/min; and temperature, 25 °C. We obtained 13.99 ± 0.42 g/L of mycelium biomass by culturing for 15 days with the optimized formulation. This was 2-fold higher than the mycelial mass obtained with the sub-optimal formulation. The extracellular fungal polysaccharides showed significant antioxidant activity against ABTS and DPPH free radicals, and significantly reduced the in vitro growth and survival of several cancer cell lines. The anticancer activity of the extracellular fungal polysaccharides was significantly higher in the human glioma cells than in other cancer cell lines. In summary, this study optimized the liquid media formulation and conditions for the large-scale culturing of Furthermore, the extracellular polysaccharides from showed significant antioxidant and anticancer activities.
是一种生长在桑树上的珍稀真菌，具有巨大的药用价值。本研究旨在通过单向试验和响应面法优化液体发酵培养基配方和培养条件，以实现大规模培养。还分析了其胞外多糖的抗氧化和抗癌活性。的最佳配方和生长条件如下：葡萄糖，30.2±0.37 g/L；酵母提取物，14.60±0.05 g/L；蒲公英粉，1.24±0.01 g/L；摇床转速，150 r/min；温度，25℃。用优化后的配方培养15天，我们获得了13.99±0.42 g/L的菌丝体生物量。这比次优配方获得的菌丝体量高出2倍。胞外真菌多糖对ABTS和DPPH自由基显示出显著的抗氧化活性，并显著降低了几种癌细胞系的体外生长和存活率。胞外真菌多糖在人胶质瘤细胞中的抗癌活性明显高于其他癌细胞系。总之，本研究优化了液体培养基配方和条件以实现大规模培养。此外，的胞外多糖显示出显著的抗氧化和抗癌活性。
