Junaid Muhammad, Lu Hongyu, Li Yixiang, Liu Yu, Din Ahmad Ud, Qi Zhongquan, Xiong Yi, Yan Jianhua
Medical College, Guangxi University, Nanning 530004, China.
Plants for Human Health Institute, North Carolina State University, 600 Laureate Way, Kannapolis, NC 28081, USA.
Genes (Basel). 2024 Mar 29;15(4):435. doi: 10.3390/genes15040435.
( a prevalent cause of foodborne infection, induces significant changes in the host transcriptome and metabolome. The lack of therapeutics with minimal or no side effects prompts the scientific community to explore alternative therapies. This study investigates the therapeutic potential of a probiotic mixture comprising () and () against , utilizing transcriptome and metabolomic analyses, a novel approach that has not been previously documented. Twenty-four SPF-BALB/c mice were divided into four groups: control negative group (CNG); positive control group (CPG); probiotic-supplemented non-challenged group (LAPG); and probiotic-supplemented -challenged group (LAPST). An RNA-sequencing analysis of small intestinal (ileum) tissue revealed 2907 upregulated and 394 downregulated DEGs in the LAPST vs. CPG group. A functional analysis of DEGs highlighted their significantly altered gene ontology (GO) terms related to metabolism, gut integrity, cellular development, and immunity ( 0.05). The KEGG analysis showed that differentially expressed genes (DEGs) in the LAPST group were primarily involved in pathways related to gut integrity, immunity, and metabolism, such as MAPK, PI3K-Akt, AMPK, the tryptophan metabolism, the glycine, serine, and threonine metabolism, ECM-receptor interaction, and others. Additionally, the fecal metabolic analysis identified 1215 upregulated and 305 downregulated metabolites in the LAPST vs. CPG group, implying their involvement in KEGG pathways including bile secretion, propanoate metabolism, arginine and proline metabolism, amino acid biosynthesis, and protein digestion and absorption, which are vital for maintaining barrier integrity, immunity, and metabolism. In conclusion, these findings suggest that the administration of a probiotic mixture improves immunity, maintains gut homeostasis and barrier integrity, and enhances metabolism in infection.
（食源性感染的常见病因，可引起宿主转录组和代谢组的显著变化。缺乏副作用极小或无副作用的治疗方法促使科学界探索替代疗法。本研究利用转录组和代谢组分析，这一此前未被记录的新方法，研究了包含（）和（）的益生菌混合物对的治疗潜力。将24只无特定病原体（SPF）-BALB/c小鼠分为四组：阴性对照组（CNG）；阳性对照组（CPG）；补充益生菌未受攻击组（LAPG）；补充益生菌受攻击组（LAPST）。对小肠（回肠）组织进行的RNA测序分析显示，与CPG组相比，LAPST组中有2907个上调和394个下调的差异表达基因（DEG）。对DEG的功能分析突出了它们与代谢、肠道完整性、细胞发育和免疫相关的显著改变的基因本体（GO）术语（P < 0.05）。KEGG分析表明，LAPST组中差异表达基因（DEG）主要参与与肠道完整性、免疫和代谢相关的途径，如丝裂原活化蛋白激酶（MAPK）、磷脂酰肌醇-3激酶-蛋白激酶B（PI3K-Akt）、腺苷酸活化蛋白激酶（AMPK）、色氨酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、细胞外基质受体相互作用等。此外，粪便代谢分析确定，与CPG组相比，LAPST组中有1215个上调和305个下调的代谢物，这意味着它们参与了KEGG途径，包括胆汁分泌、丙酸代谢、精氨酸和脯氨酸代谢、氨基酸生物合成以及蛋白质消化和吸收，这些对于维持屏障完整性、免疫和代谢至关重要。总之，这些发现表明，施用益生菌混合物可改善免疫力、维持肠道稳态和屏障完整性，并增强感染中的代谢。 ）
需注意，原文中部分括号内容缺失具体信息，翻译时保留了括号形式。
