Tang Kun-Quan, Li Miao-Yuan, Guo Qin-Yuan, Wang Juan, Lu Huo-Qing, Wang Bei
Department of Traditional Chinese Medicine, Liuzhou Women and Children's Healthcare Hospital, Liuzhou, China.
Transl Pediatr. 2024 Dec 31;13(12):2118-2133. doi: 10.21037/tp-24-257. Epub 2024 Dec 27.
Hand, foot, and mouth disease (HFMD) is a prevalent infectious condition in children. This study aimed to assess the regulatory effects of Re-Du-Ning on the intestinal microflora of pediatric patients with HFMD.
Fecal samples were collected from children affected by HFMD, who were diagnosed at the traditional Chinese medicine pediatrics outpatient and emergency departments of Liuzhou Women and Children's Healthcare Hospital, as well as from healthy children undergoing physical examinations at the same hospital during the same period. DNA was extracted from these samples and subjected to 16S ribosome DNA amplicon sequencing. The sequenced data were categorized, quantified, and compared. Analyses involved creating relative abundance bar graphs, constructing unweighted pair-group method with arithmetic mean clustering trees, and generating heatmaps of clustering to evaluate the variations in abundance and diversity across different groups. The analysis of molecular variance and -test were used to analyze structural differences in microbial flora between groups, and linear discriminant analysis was used to identify significant differences in microbial genera between the groups.
A total of 67 fecal samples were collected from children with HFMD (13 in the intravenous group, 40 in the enema group) and from healthy children (14 in the healthy group). When compared with the healthy group, the intestinal microflora diversity and similarity were highest after enema treatment, although the microbial structure exhibited significant changes (weighted_unifrac, P<0.05). The composition of species relative abundance was comparable between the healthy group and the post-enema group.
Re-Du-Ning enema treatment regulated the intestinal microflora in these children, significantly increasing the abundance of probiotics like and reducing the abundance of opportunistic pathogens like .
手足口病（HFMD）是儿童中一种常见的传染病。本研究旨在评估热毒宁对手足口病患儿肠道菌群的调节作用。
从柳州市妇幼保健院中医儿科门诊和急诊科诊断为手足口病的患儿以及同期在该院进行体检的健康儿童中采集粪便样本。从这些样本中提取DNA并进行16S核糖体DNA扩增子测序。对测序数据进行分类、定量和比较。分析包括创建相对丰度柱状图、构建算术平均非加权组对法聚类树以及生成聚类热图，以评估不同组间丰度和多样性的变化。采用分子方差分析和t检验分析组间微生物菌群的结构差异，并用线性判别分析确定组间微生物属的显著差异。
共从手足口病患儿（静脉组13例，灌肠组40例）和健康儿童（健康组14例）中采集了67份粪便样本。与健康组相比，灌肠治疗后肠道菌群多样性和相似性最高，尽管微生物结构发生了显著变化（加权UniFrac，P<0.05）。健康组和灌肠后组之间物种相对丰度的组成相当。
热毒宁灌肠治疗调节了这些儿童的肠道菌群，显著增加了如[具体益生菌名称]等益生菌的丰度，并降低了如[具体机会致病菌名称]等机会致病菌的丰度。
