Kapoor Shilpa, Yang Ying Ting, Hall Robyn N, Gasser Robin B, Bowles Vernon M, Perry Trent, Anstead Clare A
Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC 3010, Australia.
Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, VIC 3010, Australia.
Genes (Basel). 2024 Apr 18;15(4):506. doi: 10.3390/genes15040506.
The Australian sheep blowfly, , is a major sheep ectoparasite causing subcutaneous myiasis (flystrike), which can lead to reduced livestock productivity and, in severe instances, death of the affected animals. It is also a primary colonizer of carrion, an efficient pollinator, and used in maggot debridement therapy and forensic investigations. In this study, we report the complete mitochondrial (mt) genome of from the Northern Territory (NT), Australia, where sheep are prohibited animals, unlike the rest of Australia. The mt genome is 15,943 bp in length, comprising 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and a non-coding control region. The gene order of the current mt genome is consistent with the previously published mt genomes. Nucleotide composition revealed an AT bias, accounting for 77.5% of total mt genome nucleotides. Phylogenetic analyses of 56 species/taxa of dipterans indicated that and are the closest among all sibling species of the genus , which helps to explain species evolution within the family Luciliinae. This study provides the first complete mt genome sequence for derived from the NT, Australia to facilitate species identification and the examination of the evolutionary history of these blowflies.
澳大利亚羊蝇是一种主要的绵羊体外寄生虫，可导致皮下蝇蛆病（羊蝇蛆症），这会降低牲畜生产力，在严重情况下，还会导致受感染动物死亡。它也是腐肉的主要定殖者、高效传粉者，并用于蛆虫清创疗法和法医调查。在本研究中，我们报告了来自澳大利亚北领地（NT）的澳大利亚羊蝇的完整线粒体（mt）基因组，在北领地，与澳大利亚其他地区不同，绵羊是违禁动物。该mt基因组长度为15943 bp，包括13个蛋白质编码基因（PCGs）、两个核糖体RNA（rRNAs）、22个转运RNA（tRNAs）和一个非编码控制区。当前mt基因组的基因顺序与先前发表的澳大利亚羊蝇mt基因组一致。核苷酸组成显示出AT偏好，占mt基因组总核苷酸的77.5%。对56种双翅目物种/分类单元的系统发育分析表明，在丽蝇属的所有姊妹物种中，澳大利亚羊蝇和某物种最为接近，这有助于解释丽蝇亚科内的物种进化。本研究提供了首个源自澳大利亚北领地的澳大利亚羊蝇的完整mt基因组序列，以促进物种鉴定和对这些羊蝇进化历史的研究。
