双翅目昆虫线粒体基因组的全序列特征及其系统发育意义

Characterization and Phylogenetic Implications of the Complete Mitochondrial Genome of Syrphidae.

机构信息

Shaanxi Key Laboratory of Bio-Resources, School of Biological Science & Engineering, Shaanxi University of Technology, Hanzhong 723000, China.

College of Life Sciences, Northwest University, Xi'an 710069, China.

出版信息

Genes (Basel). 2019 Jul 25;10(8):563. doi: 10.3390/genes10080563.

In this study, the complete mitochondrial genomes (mitogenomes) of two hoverfly species of (Huo, Ren, and Zheng) and Coquillett (Diptera: Syrphidae) were determined and analyzed. The circular mitogenomes were 16,473 bp in (GenBank No. MK870078) and 15,724 bp in (GenBank No. MK870079). Two newly sequenced mitogenomes both contained 37 genes, and the gene order was similar with other syrphine species. All the protein-coding genes (PCGs) were started with the standard ATN codons; and most of PCGs were terminated with a TAA stop codon, while in ended with a TAG codon, and terminated with truncated T stop codons in both species. The phylogenetic relationship between and with related lineages was reconstructed using Bayesian inference and Maximum-likelihood analyses. The monophyly of each family considered within Muscomorpha was confirmed by the clades in the phylogenetic tree, and superfamily of the Oestroidea (Calliphoridae, Sarcophagidae, and Oestridae) was unexpectedly found to be a paraphyletic group based on our selected data. This mitogenome information for and could facilitate future studies of evolutionarily related insects.

在这项研究中，测定并分析了两种食蚜蝇科（双翅目）的完整线粒体基因组（线粒体基因组），即（Huo、Ren 和 Zheng）和（Coquillett）。圆形线粒体基因组在（GenBank No. MK870078）中为 16473bp，在（GenBank No. MK870079）中为 15724bp。这两个新测序的线粒体基因组都包含 37 个基因，基因排列与其他食蚜蝇科物种相似。所有的蛋白质编码基因（PCGs）都以标准的 ATN 密码子开始；大多数 PCGs 以 TAA 终止密码子终止，而在中以 TAG 密码子终止，在两个物种中均以截短的 T 终止密码子终止。利用贝叶斯推断和最大似然分析重建了与相关谱系之间的系统发育关系。在系统发育树中的分支证实了 Muscomorpha 内每个科的单系性，并且基于我们选择的数据，出乎意料地发现 Oestroidea 超科（Calliphoridae、Sarcophagidae 和 Oestridae）是一个并系群。本研究提供的和线粒体基因组信息有助于未来对进化相关昆虫的研究。