Maeda Satoru, Goto Shingo, Inoue Haruhiko, Suwazono Haruka, Takatsuji Hiroshi, Mori Masaki
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba 305-8634, Japan.
Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization, Shizuoka 424-0292, Japan.
Plants (Basel). 2024 Apr 18;13(8):1138. doi: 10.3390/plants13081138.
Conferring crops with resistance to multiple diseases is crucial for stable food production. Genetic engineering is an effective means of achieving this. The rice receptor-like cytoplasmic kinase BSR1 mediates microbe-associated molecular pattern-induced immunity. In our previous study, we demonstrated that rice lines overexpressing under the control of the maize ubiquitin promoter exhibited broad-spectrum resistance to rice blast, brown spot, leaf blight, and bacterial seedling rot. However, unfavorable phenotypes were observed, such as a decreased seed germination rate and a partial darkening of husked rice. Herein, we present a strategy to address these unfavorable phenotypes using an constitutive promoter with moderate expression levels and a pathogen-inducible promoter. Rice lines expressing under the influence of both promoters maintained broad-spectrum disease resistance. The seed germination rate and coloration of husked rice were similar to those of the wild-type rice.
赋予作物对多种病害的抗性对于稳定粮食生产至关重要。基因工程是实现这一目标的有效手段。水稻类受体细胞质激酶BSR1介导微生物相关分子模式诱导的免疫反应。在我们之前的研究中，我们证明了在玉米泛素启动子控制下过表达的水稻株系对稻瘟病、褐斑病、白叶枯病和细菌性苗腐病表现出广谱抗性。然而，观察到了不利的表型，如种子发芽率降低和糙米部分变黑。在此，我们提出一种策略，使用具有中等表达水平的组成型启动子和病原体诱导型启动子来解决这些不利表型。在两种启动子影响下表达的水稻株系保持了广谱抗病性。种子发芽率和糙米颜色与野生型水稻相似。
