Institute of Agrobiological Sciences, NARO (NIAS), Tsukuba 305-8634, Japan.
Department of Applied Biological Science, Graduate School of Science and Technology, Tokyo University of Science, Noda 278-8510, Japan.
Int J Mol Sci. 2023 Jun 20;24(12):10395. doi: 10.3390/ijms241210395.
Crops experience herbivory by arthropods and microbial infections. In the interaction between plants and chewing herbivores, lepidopteran larval oral secretions (OS) and plant-derived damage-associated molecular patterns (DAMPs) trigger plant defense responses. However, the mechanisms underlying anti-herbivore defense, especially in monocots, have not been elucidated. The receptor-like cytoplasmic kinase Broad-Spectrum Resistance 1 (BSR1) of L. (rice) mediates cytoplasmic defense signaling in response to microbial pathogens and enhances disease resistance when overexpressed. Here, we investigated whether BSR1 contributes to anti-herbivore defense responses. knockout suppressed rice responses triggered by OS from the chewing herbivore Duponchel (Lepidoptera: Noctuidae) and peptidic DAMPs OsPeps, including the activation of genes required for biosynthesis of diterpenoid phytoalexins (DPs). -overexpressing rice plants exhibited hyperactivation of DP accumulation and ethylene signaling after treatment with simulated herbivory and acquired enhanced resistance to larval feeding. As the biological significance of herbivory-induced accumulation of rice DPs remains unexplained, their physiological activities in were analyzed. The addition of momilactone B, a rice DP, to the artificial diet suppressed the growth of larvae. Altogether, this study revealed that BSR1 and herbivory-induced rice DPs are involved in the defense against chewing insects, in addition to pathogens.
作物受到节肢动物和微生物感染的侵害。在植物与咀嚼性食草动物的相互作用中，鳞翅目幼虫的口腔分泌物（OS）和植物来源的损伤相关分子模式（DAMPs）引发植物防御反应。然而，抗食草动物防御的机制，特别是在单子叶植物中，尚未阐明。 L.（水稻）的类受体细胞质激酶广谱抗性 1（BSR1）介导对微生物病原体的细胞质防御信号转导，并在过表达时增强抗病性。在这里，我们研究了 BSR1 是否有助于抗食草动物防御反应。 BSR1 缺失抑制了咀嚼性食草动物 Duponchel（鳞翅目：夜蛾科）的 OS 和肽 DAMPs OsPeps 触发的水稻反应，包括二萜类植物抗毒素（DPs）生物合成所需基因的激活。过表达水稻植物在模拟食草后表现出 DP 积累和乙烯信号的过度激活，并获得对幼虫取食的增强抗性。由于食草诱导的水稻 DPs 积累的生物学意义尚不清楚，因此分析了它们在中的生理活性。向人工饲料中添加 momilactone B（一种水稻 DP）可抑制幼虫的生长。总之，这项研究表明，BSR1 和食草诱导的水稻 DPs 除了参与防御病原体外，还参与了对咀嚼性昆虫的防御。
