白蛉（ Psychodidae: Phlebotominae）对杜氏利什曼原虫（Leishmania donovani）和婴儿利什曼原虫（Leishmania infantum）（锥虫科：利什曼原虫亚科）的实验易感性。

Experimental Susceptibility of and (Psychodidae: Phlebotominae) to () and () (Trypanosomatidae: Leishmaniinae).

作者信息

Sánchez Uzcátegui Yetsenia Del Valle, Silveira Fernando Tobias, de Morais Thais Gouvea, Furtado Rodrigo Ribeiro, Vasconcelos Dos Santos Thiago, Póvoa Marinete Marins

机构信息

Programa de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-110, PA, Brazil.

Seção de Parasitologia, Instituto Evandro Chagas, Ananindeua 67030-000, PA, Brazil.

出版信息

Microorganisms. 2024 Apr 17;12(4):809. doi: 10.3390/microorganisms12040809.

The present work assessed the experimental susceptibility of and to () and (.) . A () - combination was used as a susceptible control. Wild-caught and laboratory-bred were membrane-fed on blood with a 5 × 10/mL log-phase promastigote culture suspension and dissected on days 2 and 8 post-blood meal (pbm) for analysis focused on the assessment of parasitoses, as well as placement and promastigote morphotyping. Survival curves were constructed. In all combinations, promastigotes were observed on day 8 pbm. For both species, in , the presence of parasites was observed up to the stomodeal valve, while in , the presence of parasites was observed up to the cardia. There were no significant differences in parasitosis between () and () in either or . Six morphological promastigote forms were distinguished in Giemsa-stained gut smears. The survival curves of all combinations decreased and were affected differently by several -parasite combinations, as well with -uninfected blood. These findings stress as experimentally susceptible to spp. and suggest the putative susceptibility of to () and () .

本研究评估了[具体物种1]和[具体物种2]对[寄生虫1]（[寄生虫1学名]）和[寄生虫2]（[寄生虫2学名]）的实验易感性。使用[具体组合]作为易感对照。对野生捕获的[具体物种1]和实验室饲养的[具体物种2]进行膜饲含有5×10⁶/mL对数期前鞭毛体培养悬浮液的血液，并在血餐（pbm）后第2天和第8天解剖，分析重点在于评估寄生虫感染情况以及前鞭毛体的定位和形态分型。构建了生存曲线。在所有组合中，在pbm第8天均观察到前鞭毛体。对于这两个[具体物种]，在[具体物种1]中，在口道瓣膜处均观察到寄生虫存在，而在[具体物种2]中，在贲门处观察到寄生虫存在。在[具体物种1]或[具体物种2]中，[寄生虫1]（[寄生虫1学名]）和[寄生虫2]（[寄生虫2学名]）之间的寄生虫感染情况没有显著差异。在吉姆萨染色的肠道涂片中共区分出六种形态的前鞭毛体。所有组合的生存曲线均下降，并且受到几种寄生虫 - 宿主组合以及未感染血液的不同影响。这些发现强调[具体物种1]在实验上对[寄生虫1]属物种易感，并表明[具体物种2]可能对[寄生虫1]（[寄生虫1学名]）和[寄生虫2]（[寄生虫2学名]）易感。