Jeencham Rachasit, Sinna Jiraporn, Ruksakulpiwat Chaiwat, Tawonsawatruk Tulyapruek, Numpaisal Piya-On, Ruksakulpiwat Yupaporn
Research Center for Biocomposite Materials for Medical Industry and Agricultural and Food Industry, Nakhon Ratchasima 30000, Thailand.
Institute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Polymers (Basel). 2024 Apr 14;16(8):1093. doi: 10.3390/polym16081093.
The development of a hydrogel material with a modified chemical structure of poly(vinyl alcohol) (PVA) and silk fibroin (SF) using glycidyl methacrylate (GMA) (denoted as PVA-g-GMA and SF-g-GMA) is an innovative approach in the field of biomaterials and meniscus tissue engineering in this study. The PVA-g-GMA/SF-g-GMA hydrogel was fabricated using different ratios of PVA-g-GMA to SF-g-GMA: 100/0, 75/25, 50/50, 25/75, and 0/100 (/ of dry substances), using lithium phenyl (2,4,6-trimethylbenzoyl)phosphinate (LAP) as a free radical photoinitiator, for 10 min at a low ultraviolet (UV) intensity (365 nm, 6 mW/cm). The mechanical properties, morphology, pore size, and biodegradability of the PVA-g-GMA/SF-g-GMA hydrogel were investigated. Finally, for clinical application, human chondrocyte cell lines (HCPCs) were mixed into PVA-g-GMA/SF-g-GMA solutions and fabricated into hydrogel to study the viability of live and dead cells and gene expression. The results indicate that as the SF-g-GMA content increased, the compressive modulus of the PVA-g-GMA/SF-g-GMA hydrogel dropped from approximately 173 to 11 kPa. The degradation rates of PVA-g-GMA/SF-g-GMA 100/0, 75/25, and 50/50 reached up to 15.61%, 17.23%, and 18.93% in 4 months, respectively. In all PVA-g-GMA/SF-g-GMA conditions on day 7, chondrocyte cell vitality exceeded 80%. The PVA-g-GMA/SF-g-GMA 75:25 and 50:50 hydrogels hold promise as a biomimetic biphasic injectable hydrogel for encapsulated augmentation, offering advantages in terms of rapid photocurability, tunable mechanical properties, favorable biological responses, and controlled degradation.
本研究中，使用甲基丙烯酸缩水甘油酯（GMA）（分别记为PVA-g-GMA和SF-g-GMA）对聚乙烯醇（PVA）和丝素蛋白（SF）的化学结构进行改性，从而开发一种水凝胶材料，这是生物材料和半月板组织工程领域的一种创新方法。使用不同比例的PVA-g-GMA与SF-g-GMA（干物质比例为100/0、75/25、50/50、25/75和0/100），以苯基（2,4,6-三甲基苯甲酰基）膦酸锂（LAP）作为自由基光引发剂，在低紫外线（UV）强度（365 nm，6 mW/cm）下照射10分钟，制备PVA-g-GMA/SF-g-GMA水凝胶。对PVA-g-GMA/SF-g-GMA水凝胶的力学性能、形态、孔径和生物降解性进行了研究。最后，为用于临床，将人软骨细胞系（HCPCs）混入PVA-g-GMA/SF-g-GMA溶液中并制成水凝胶，以研究活细胞和死细胞的活力以及基因表达。结果表明，随着SF-g-GMA含量的增加，PVA-g-GMA/SF-g-GMA水凝胶的压缩模量从约173 kPa降至11 kPa。PVA-g-GMA/SF-g-GMA 100/0、75/25和50/50在4个月内的降解率分别达到15.61%、17.23%和18.93%。在第7天的所有PVA-g-GMA/SF-g-GMA条件下，软骨细胞活力均超过80%。PVA-g-GMA/SF-g-GMA 75:25和50:50水凝胶有望作为一种用于包裹性增强的仿生双相可注射水凝胶，在快速光固化、可调机械性能、良好的生物学反应和可控降解方面具有优势。
