Department of Mechanics of Biosystem Engineering, Faculty of Engineering & Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj, Alborz, Iran.
Department of Food Technology, College of Aburaihan, University of Tehran, Tehran, Iran.
J Biotechnol. 2022 Sep 20;357:56-83. doi: 10.1016/j.jbiotec.2022.08.003. Epub 2022 Aug 13.
The temporary immersion systems (TISs) have been widely used in plant biotechnology. TISs have different advantages from the point of micropropagation and production of secondary metabolites over other continuous liquid-phase bioreactors. The current work presents the structure, operation mode, configuration type, and micropropagation or secondary metabolite production in TISs. This review deals with the advantages and disadvantages of TISs and the factors affecting their performance. Future research could focus on new designs based on CFD simulation, facilitating sterilization, and combining TISs with other bioreactors (e.g., mist bioreactors) to make a hybrid bioreactor.
临时沉浸系统（TISs）已在植物生物技术中得到广泛应用。从微繁殖和次生代谢产物生产的角度来看，TISs 相对于其他连续液相生物反应器具有不同的优势。目前的工作介绍了 TISs 的结构、操作模式、配置类型，以及在 TISs 中的微繁殖或次生代谢产物生产。本综述讨论了 TISs 的优缺点以及影响其性能的因素。未来的研究可以集中在基于 CFD 模拟的新设计、便于灭菌以及将 TISs 与其他生物反应器（例如，雾生物反应器）结合以制造混合生物反应器上。
