Rico Saleta, Garrido José, Sánchez Conchi, Ferreiro-Vera Carlos, Codesido Verónica, Vidal Nieves
Misión Biológica de Galicia- Sede Santiago de Compostela, MBG-CSIC, Departamento Producción Vegetal, Santiago de Compostela, Spain.
Phytoplant Research S.L.U, Departamento Hibridación y Cultivo, Rabanales 21-Parque Científico Tecnológico de Córdoba, Calle Astrónoma Cecilia Payne, Córdoba, Spain.
Front Plant Sci. 2022 Jun 23;13:895971. doi: 10.3389/fpls.2022.895971. eCollection 2022.
The aim of this study was to propagate axillary shoots of L. using liquid medium in temporary immersion bioreactors. The effect of immersion frequency (3 or 6 immersions per day), explant type (apical or basal sections), explant number (8, 10, and 16 explants), mineral medium (Murashige and Skoog half-strength nitrates, -A and -H, all supplemented with 2-μM metatopoline), sucrose supplementation (2, 0.5, and 0% sucrose), culture duration (4 and 6 weeks), and bioreactor type (RITA® and Plantform™) were investigated. As a result, we propose a protocol for the proliferation of cannabis apical segments in RITA® or Plantform™ bioreactors. The explants (8 per RITA® and 24 per Plantform™) are immersed for 1 min, 3 times per day in -A medium supplemented with 2-μM metatopoline and 0.5% of sucrose and subcultured every 4 weeks. This is the first study using temporary immersion systems in production, and our results provide new opportunities for the mass propagation of this species.
本研究的目的是在临时浸没式生物反应器中使用液体培养基繁殖大麻的腋芽。研究了浸没频率（每天3次或6次浸没）、外植体类型（顶端或基部切段）、外植体数量（8、10和16个外植体）、矿物培养基（Murashige和Skoog半强度硝酸盐、-A和-H，均添加2 μM间苯三酚）、蔗糖添加量（2%、0.5%和0%蔗糖）、培养持续时间（4周和6周）以及生物反应器类型（RITA®和Plantform™）的影响。结果，我们提出了一种在RITA®或Plantform™生物反应器中繁殖大麻顶端切段的方案。外植体（每个RITA® 8个，每个Plantform™ 24个）每天浸没3次，每次1分钟，置于添加2 μM间苯三酚和0.5%蔗糖的-A培养基中，每4周继代培养一次。这是首次在大麻生产中使用临时浸没系统的研究，我们的结果为该物种的大规模繁殖提供了新的机会。
