基于适配体的金纳米颗粒侧向流动试纸条用于检测冷链食品包装表面的SARS-CoV-2 S蛋白的研究进展
The Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for the Detection of SARS-CoV-2 S Proteins on the Surface of Cold-Chain Food Packaging.
作者信息
Li Xiaotong, Wang Jiachen, Yang Ge, Fang Xiaona, Zhao Lianhui, Luo Zhaofeng, Dong Yiyang
机构信息
Laboratory of Food Safety and Risk Assessment, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
CAMS Key Laboratory of Antiviral Drug Research, Beijing Key Laboratory of Antimicrobial Agents, NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
出版信息
Molecules. 2024 Apr 13;29(8):1776. doi: 10.3390/molecules29081776.
The COVID-19 pandemic over recent years has shown a great need for the rapid, low-cost, and on-site detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, an aptamer-based colloidal gold nanoparticle lateral flow test strip was well developed to realize the visual detection of wild-type SARS-CoV-2 spike proteins (SPs) and multiple variants. Under the optimal reaction conditions, a low detection limit of SARS-CoV-2 S proteins of 0.68 nM was acquired, and the actual detection recovery was 83.3% to 108.8% for real-world samples. This suggests a potential tool for the prompt detection of SARS-CoV-2 with good sensitivity and accuracy, and a new method for the development of alternative antibody test strips for the detection of other viral targets.
近年来的新冠疫情表明，对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）进行快速、低成本和现场检测的需求极为迫切。在本研究中，一种基于适配体的胶体金纳米颗粒侧向流动测试条被成功开发出来，以实现对野生型SARS-CoV-2刺突蛋白（SPs）和多种变体的可视化检测。在最佳反应条件下，获得了SARS-CoV-2 S蛋白0.68 nM的低检测限，实际检测回收率在实际样本中为83.3%至108.8%。这表明该测试条是一种具有良好灵敏度和准确性的快速检测SARS-CoV-2的潜在工具，也是开发用于检测其他病毒靶点的替代抗体测试条的新方法。
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