Petrini Stefano, Brutti Andrea, Casciari Cristina, Calderone Davide, Pela Michela, Giammarioli Monica, Righi Cecilia, Feliziani Francesco
National Reference Centre for Pestiviruses and Asfivirus, Istituto Zooprofilattico Sperimentale Umbria-Marche "Togo Rosati", Via Gaetano Salvemini, 1, 06126 Perugia, Italy.
SSICA Stazione Sperimentale per l'Industria delle Conserve Alimentari, Fondazione di Ricerca Parma, 43121 Parma, Italy.
Viruses. 2024 Apr 19;16(4):638. doi: 10.3390/v16040638.
African swine fever (ASF) is a disease that is a growing threat to the global swine industry. Regulations and restrictions are placed on swine movement to limit the spread of the virus. However, these are costly and time-consuming. Therefore, this study aimed to determine if high-pressure processing (HPP) sanitization techniques would be effective against the ASF virus. Here, it was hypothesized that HPP could inactivate or reduce ASF virus infectivity in tissue homogenates. To test this hypothesis, 30 aliquots of each homogenate (spleen, kidney, loin) were challenge-infected with the Turin/83 strain of ASF, at a 10 median hemadsorption dose (HAD)/mL. Subsequently, eight aliquots of each homogenate were treated with 600 millipascal (600 MPa) HPP for 3, 5, and 7 min. Six untreated aliquots were used as the controls. Virological results showed a reduction in the viral titer of more than 7-log. These results support the validity of the study hypothesis since HPP treatment was effective in inactivating ASFV in artificially prepared samples. Overall, this study suggests the need for further investigation of other ASFV-contaminated meat products.
非洲猪瘟（ASF）是一种对全球养猪业构成日益严重威胁的疾病。对猪的移动实施了法规和限制措施，以限制病毒的传播。然而，这些措施成本高昂且耗时。因此，本研究旨在确定高压处理（HPP）消毒技术对非洲猪瘟病毒是否有效。在此，研究假设高压处理能够使组织匀浆中的非洲猪瘟病毒失活或降低其感染力。为验证这一假设，将每种匀浆（脾脏、肾脏、里脊肉）的30份等分试样用都灵/83株非洲猪瘟病毒以10个半数血细胞吸附剂量（HAD）/毫升进行攻击感染。随后，将每种匀浆的8份等分试样分别用600毫帕斯卡（600兆帕）的高压处理3分钟、5分钟和7分钟。6份未处理的等分试样用作对照。病毒学结果显示病毒滴度降低了7个对数以上。这些结果支持了研究假设的有效性，因为高压处理能有效使人工制备样本中的非洲猪瘟病毒失活。总体而言，本研究表明有必要对其他受非洲猪瘟病毒污染的肉制品进行进一步研究。
