Almhanna Hazem, Kumar Arun Hs, Kilroy David, Duggan Gina, Irwin Jane A, Hogg Bridget, Reid Colm
Department of Anatomy and Histology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
School of Veterinary Medicine, University College Dublin, Belfield, Dublin-04, Ireland.
Vet World. 2024 Mar;17(3):645-657. doi: 10.14202/vetworld.2024.645-657. Epub 2024 Mar 21.
Sialic acid-binding immunoglobulin-like lectin 1 (Siglec-1) is a transmembrane glycoprotein involved in the sialic acid (Sia)-dependent regulation of the immune system. Siglec-1 expression has recently been identified in the male reproductive tract (MRT) of several species, including humans, cattle, horses, and sheep, and may play a role in modulating fertility in a Sia-dependent manner.
In this study, protein-protein interaction (PPI) analysis of Siglec-1 was conducted to identify associated network protein conservation, and the expression of Siglec-1 in the MRT of mice and rats, including their accessory sex glands and spermatozoa was determined by immunostaining.
Network analysis of proteins with Siglec-1 in mice and rats demonstrated significant similarity to human Siglec-1 networks, suggesting a similar conservation of network proteins between these species and, hence, a potential conservation role in immune modulation and function. Specific immunostaining patterns of mouse and rat testes, epididymis, ductus deferens, accessory sex gland tissues, and sperm were detected using human Siglec-1. These results confirmed that the human Siglec-1 antibody could cross-react with mouse and rat Siglec-1, suggesting that the specific expression patterns of Siglec-1 in the MRT and sperm of both mice and rats are similar to those observed in other species.
The conservation of Siglec-1 expression patterns in sperm and within the MRT and the similarity of protein networks for Siglec-1 across species suggest that Siglec-1 may function in a similar manner across species. These results also suggest that rodents may serve as a valuable model system for exploring the function of Siglecs in the reproductive system across species and their potential role in modulating fertility in a Sia-dependent manner.
唾液酸结合免疫球蛋白样凝集素1（Siglec-1）是一种跨膜糖蛋白，参与唾液酸（Sia）依赖性的免疫系统调节。最近在包括人类、牛、马和羊在内的几种物种的雄性生殖道（MRT）中发现了Siglec-1的表达，并且它可能以Sia依赖性方式在调节生育能力方面发挥作用。
在本研究中，对Siglec-1进行了蛋白质-蛋白质相互作用（PPI）分析以确定相关网络蛋白的保守性，并通过免疫染色确定了Siglec-1在小鼠和大鼠的MRT（包括其附属性腺和精子）中的表达。
对小鼠和大鼠中与Siglec-1相互作用的蛋白质进行网络分析，结果表明与人类Siglec-1网络具有显著相似性，这表明这些物种之间的网络蛋白具有相似的保守性，因此在免疫调节和功能方面可能具有潜在的保守作用。使用人Siglec-1检测到了小鼠和大鼠睾丸、附睾、输精管、附属性腺组织和精子的特异性免疫染色模式。这些结果证实人Siglec-1抗体可与小鼠和大鼠的Siglec-1发生交叉反应，这表明Siglec-1在小鼠和大鼠的MRT及精子中的特异性表达模式与在其他物种中观察到的相似。
Siglec-1在精子和MRT中的表达模式的保守性以及跨物种的Siglec-1蛋白质网络的相似性表明，Siglec-1可能在不同物种中以相似的方式发挥作用。这些结果还表明，啮齿动物可能是探索Siglecs在跨物种生殖系统中的功能及其以Sia依赖性方式调节生育能力的潜在作用的有价值的模型系统。
