Mota Joana, Faria-Silva Catarina, Resendes Ana, Santos Maria Isabel, Carvalheiro Manuela Colla, Lima Ana, Simões Sandra
Faculty of Veterinary Medicine, Lusófona University, Lisbon, Portugal.
LEAF-Linking Landscape, Environment, Agriculture and Food Research Center, Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, Lisboa, Portugal.
Nat Prod Res. 2024 Apr 29:1-12. doi: 10.1080/14786419.2024.2347452.
Milk thistle () is well-known for its antioxidant activity due to the presence of silymarin. Albeit some studies show a potential for skin inflammation, its activity against dermal MMP-9 and MMP-2 remains to be studied. Silymarin isolated from an herbal extract was tested for gelatinase inhibition in the presence of isolated MMP-9 and in dermal adenocarcinome HaCaT cells. Silymarin was then further tested , using a cutaneous inflammation mice model mediated by reactive oxygen species. Silymarin was able to significantly inhibit gelatinolytic activity without impairing cell growth and viability. Furthermore, inhibition was more pronounced in cells than with the isolated gelatinase, suggesting an additional effect upon metabolic pathways. , silymarin was able to reduce ear edema up to 74% and attenuated histological lesions. Results highlight silymarin potential for application in skin inflammatory disorders gelatinase inhibition.
水飞蓟因其含有水飞蓟素而具有抗氧化活性而闻名。尽管一些研究表明它有引发皮肤炎症的可能性，但其对皮肤基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶-2（MMP-2）的作用仍有待研究。从一种草药提取物中分离出的水飞蓟素在分离的MMP-9存在的情况下以及在皮肤腺癌HaCaT细胞中进行了明胶酶抑制测试。然后，使用由活性氧介导的皮肤炎症小鼠模型对水飞蓟素进行了进一步测试。水飞蓟素能够显著抑制明胶分解活性，而不损害细胞生长和活力。此外，在细胞中的抑制作用比在分离的明胶酶中更明显，这表明对代谢途径有额外的影响。此外，水飞蓟素能够将耳部水肿减轻高达74%，并减轻组织学损伤。结果突出了水飞蓟素在皮肤炎症性疾病中应用的潜力，因为它具有明胶酶抑制作用。
