Omaña-Molina Maritza A, González-Robles Arturo, Salazar-Villatoro Lizbeth, Bernal-Escobar Alexander, Durán-Díaz Angel, Méndez-Cruz Adolfo René, Martínez-Palomo Adolfo
School of Superior Studies Iztacala (M.A.O-M., A.B-E., A.R.M-C.), Medicine, Universidad Nacional Autónoma de México, Los Reyes Iztacala, Tlalnepantla, Mexico; Department of Infectomics and Molecular Pathogenesis (A.G-R., L.S-V., A.M-P.), Center for Research and Advanced Studies, Mexico City, Mexico; and School of Superior Studies Iztacala (A.D-D.), Biology, Universidad Nacional Autónoma de México, Los Reyes Iztacala, Tlalnepantla, Mexico.
Eye Contact Lens. 2014 May;40(3):132-9. doi: 10.1097/ICL.0000000000000024.
To describe the adhesion properties of Acanthamoeba castellanii trophozoites to silicone hydrogel contact lenses of first generation (lotrafilcon A), second generation (galyfilcon A), and third generation (comfilcon A) and correlate the results with their specific surface characteristics, time of interaction, and suspension media.
Qualitative and quantitative assessments of the adhesion of 200 trophozoites of A. castellanii on contact lenses in culture medium (Bacto Casitone) and isotonic saline (IS) at different time points (15 minutes and 6 hours) were determined.
By scanning electron microscopy, A. castellanii trophozoites were observed firmly adhered to the surface of hydrogel lenses after 15 minutes of interaction. The surface of lotrafilcon A lenses on which amoebae adhere better (16.4±10.2 amoebae/lens section) is rough and folded, which increases the contact surface with trophozoites, allowing acanthopodia to attach firmly. Contrarily, galyfilcon A lenses have a smoother surface, and lower numbers of amoebae were observed adhered to these lenses (4.7±2.9 amoebae/lens section). Even fewer amoebae adhered to the smoother surface of the comfilcon A lens (2.2±1.7 amoebae/lens section). Trophozoites showed similar behavior in both Bacto Casitone medium and IS.
A rough surface may contribute to better adhesion of amoebae to silicone hydrogel lenses. Although a reduced numbers of trophozoites adhered to smooth lenses, trophozoites are a risk factor for amoebic keratitis. Isotonic saline facilitated trophozoite survival, suggesting that homemade saline solutions may contribute to the persistence of trophozoites, especially when there is no proper hygiene regimen used with the contact lens cases.
描述卡氏棘阿米巴滋养体对第一代（lotrafilcon A）、第二代（galyfilcon A）和第三代（comfilcon A）硅水凝胶隐形眼镜的黏附特性，并将结果与其特定表面特征、相互作用时间和悬浮介质相关联。
测定200个卡氏棘阿米巴滋养体在不同时间点（15分钟和6小时）于培养基（细菌胰酪胨）和等渗盐水（IS）中对隐形眼镜的黏附的定性和定量评估。
通过扫描电子显微镜观察，相互作用15分钟后，卡氏棘阿米巴滋养体牢固地黏附在水凝胶镜片表面。阿米巴黏附较好的lotrafilcon A镜片表面（16.4±10.2个阿米巴/镜片切片）粗糙且有褶皱，这增加了与滋养体的接触面积，使伪足能够牢固附着。相反，galyfilcon A镜片表面较光滑，观察到黏附在这些镜片上的阿米巴数量较少（4.7±2.9个阿米巴/镜片切片）。黏附在comfilcon A镜片更光滑表面上的阿米巴甚至更少（2.2±1.7个阿米巴/镜片切片）。滋养体在细菌胰酪胨培养基和IS中表现出相似的行为。
粗糙表面可能有助于阿米巴更好地黏附于硅水凝胶镜片。尽管黏附在光滑镜片上的滋养体数量减少，但滋养体仍是阿米巴性角膜炎的一个危险因素。等渗盐水促进了滋养体的存活，这表明自制盐水溶液可能有助于滋养体的持续存在，尤其是在隐形眼镜盒未采用适当卫生方案的情况下。
