Faculty of Biology, Medicine and Health, The University of Manchester, Manchester, United Kingdom.
Faculty of Health Sciences, Centre for Optometry Studies, Universiti Teknologi MARA (UiTM), Selangor, Malaysia.
Invest Ophthalmol Vis Sci. 2024 May 1;65(5):2. doi: 10.1167/iovs.65.5.2.
To identify compositional differences in the gut microbiome of nonmyopes (NM) and myopes using 16S ribosomal RNA sequencing and to investigate whether the microbiome may contribute to the onset or progression of the condition.
Faecal samples were collected from 52 adult participants, of whom 23 were NM, 8 were progressive myopes (PM), and 21 were stable myopes (SM). The composition of the gut microbiota in each group was analysed using 16S ribosomal RNA gene sequencing.
There were no significant differences in alpha and beta diversity between the three groups (NM, PM, and SM). However, the distributions of Bifidobacterium, Bacteroides, Megamonas, Faecalibacterium, Coprococcus, Dorea, Roseburia, and Blautia were significantly higher in the myopes (SM and PM combined) when compared with emmetropes. The myopes exhibited significantly greater abundance of bacteria that are linked to the regulation of dopaminergic signalling, such as Clostridium, Ruminococcus, Bifidobacterium, and Bacteroides. Individuals with stable myopia were found to have a significantly higher proportion of Prevotella copri than those with progressive myopia. Bifidobacterium adolescentis, a gamma-aminobutyric acid (GABA)-producing bacterium, was significantly higher in all myopes than in NM and, in the comparison between SM and PM, it is significantly higher in SM. B. uniformis and B. fragilis, both GABA-producing Bacteroides, were present in relatively high abundance in all myopes and in SM compared with PM, respectively.
The presence of bacteria related to dopamine effect and GABA-producing bacteria in the gut microbiome of myopes may suggest a role of these microorganisms in the onset and progression of myopia.
通过 16S 核糖体 RNA 测序，鉴定非近视（NM）和近视者肠道微生物组的组成差异，并研究微生物组是否可能导致近视的发生或进展。
从 52 名成年参与者中收集粪便样本，其中 23 名是非近视者（NM），8 名是进展性近视者（PM），21 名是稳定型近视者（SM）。使用 16S 核糖体 RNA 基因测序分析每组肠道微生物组的组成。
三组（NM、PM 和 SM）之间的 alpha 和 beta 多样性无显著差异。然而，与正视者相比，双歧杆菌、拟杆菌、巨单胞菌、粪杆菌、粪球菌、多雷亚菌、罗氏菌和布劳特氏菌在近视者（SM 和 PM 合并）中的分布明显较高。近视者表现出与调节多巴胺信号传导相关的细菌的丰度显著增加，如梭菌、真杆菌、双歧杆菌和拟杆菌。与进展性近视者相比，稳定型近视者的普雷沃氏菌属丰度明显更高。产生 γ-氨基丁酸（GABA）的双歧杆菌在所有近视者中的含量明显高于 NM，在 SM 与 PM 的比较中，SM 中的含量明显更高。同样产生 GABA 的拟杆菌 B. adolescentis 和 B. fragilis 在所有近视者和 SM 中均比 PM 中更为丰富。
在近视者的肠道微生物组中存在与多巴胺作用相关的细菌和产生 GABA 的细菌，这可能表明这些微生物在近视的发生和发展中起作用。
