Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou, 221004, PR China.
School of Biology and Medicine, Shaanxi University of Science & Technology, Xi'an, 710021, PR China.
Anal Chim Acta. 2024 Jun 1;1306:342598. doi: 10.1016/j.aca.2024.342598. Epub 2024 Apr 15.
Carbon-based nanozymes have recently received enormous concern, however, there is still a huge challenge for inexpensive and large-scale synthesis of magnetic carbon-based "Two-in-One" mimics with both peroxidase (POD)-like and laccase-like activities, especially their potential applications in multi-mode sensing of antibiotics and neurotransmitters in biofluids. Although some progresses have been made in this field, the feasibility of biomass-derived carbon materials with both POD-like and laccase-like activities by polyatomic doping strategy is still unclear. In addition, multi-mode sensing platform can provide a more reliable result because of the self-validation, self-correction and mutual agreement. Nevertheless, the use of magnetic carbon-based nanozyme sensors for the multi-mode detection of antibiotics and neurotransmitters have not been investigated.
We herein report a shrimp shell-derived N, O-codoped porous carbon confined magnetic CuFeO nanosphere with outstanding laccase-like and POD-like activities for triple-mode sensing of antibiotic d-penicillamine (D-PA) and chloramphenicol (CPL), as well as colorimetric detection of neurotransmitters in biofluids. The magnetic CuFeO/N, O-codoped porous carbon (MCNPC) armored mimetics was successfully fabricated using a combined in-situ coordination and high-temperature crystallization method. The synthesized MCNPC composite with superior POD-like activity can be used for colorimetric/temperature/smartphone-based triple-mode detection of D-PA and CPL in goat serum. Importantly, the MCNPC nanozyme can also be used for colorimetric analysis of dopamine and epinephrine in human urine.
This work not only offered a novel strategy to large-scale, cheap synthesize magnetic carbon-based "Two-in-One" armored mimetics, but also established the highly sensitive and selective platforms for triple-mode monitoring D-PA and CPL, as well as colorimetric analysis of neurotransmitters in biofluids without any tanglesome sample pretreatment.
碳基纳米酶最近受到了极大的关注，然而，对于具有过氧化物酶（POD）样和漆酶样活性的廉价和大规模合成磁性碳基“二合一”模拟物，仍然存在巨大的挑战，特别是它们在生物流体中抗生素和神经递质的多模式传感中的潜在应用。尽管在这一领域已经取得了一些进展，但通过多原子掺杂策略制备具有 POD 样和漆酶样活性的生物质衍生碳材料的可行性仍然不清楚。此外，多模式传感平台由于具有自我验证、自我修正和相互一致的特点，可以提供更可靠的结果。然而，尚未研究基于磁性碳基纳米酶传感器对抗生素和神经递质的多模式检测。
我们在此报告了一种虾壳衍生的 N、O 共掺杂多孔碳限制的磁性 CuFeO 纳米球，具有出色的漆酶样和 POD 样活性，可用于抗生素 D-青霉素（D-PA）和氯霉素（CPL）的三模式传感，以及生物流体中神经递质的比色检测。通过原位配位和高温结晶法成功制备了磁性 CuFeO/N、O 共掺杂多孔碳（MCNPC）装甲模拟物。合成的具有卓越 POD 样活性的 MCNPC 复合材料可用于山羊血清中 D-PA 和 CPL 的比色/温度/智能手机三模式检测。重要的是，MCNPC 纳米酶还可用于人尿中多巴胺和去甲肾上腺素的比色分析。
这项工作不仅提供了一种大规模、廉价合成磁性碳基“二合一”装甲模拟物的新策略，而且还建立了用于 D-PA 和 CPL 的多模式监测以及生物流体中神经递质的比色分析的高灵敏度和选择性平台，而无需任何繁琐的样品预处理。
