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P-TDHM：开源便携式远心数字全息显微镜。

P-TDHM: Open-source portable telecentric digital holographic microscope.

作者信息

Jin Lei, Yu Ziyang, Au Aaron, Serles Peter, Wang Nan, Lant Jeremy T, Filleter Tobin, Yip Christopher M

机构信息

Institute of Biomedical Engineering, 164 College St, University of Toronto, Toronto, ON M5S 3G9, Canada.

Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.

出版信息

HardwareX. 2024 Jan 26;17:e00508. doi: 10.1016/j.ohx.2024.e00508. eCollection 2024 Mar.

DOI:10.1016/j.ohx.2024.e00508
PMID:38327674
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10847153/
Abstract

We present the design of a low-cost, portable telecentric digital holographic microscope (P-TDHM) that utilizes off-the-shelf components. We describe the system's hardware and software elements and evaluate its performance by imaging samples ranging from nano-printed targets to live HeLa cells, HEK293 cells, and via both in-line and off-axis modes. Our results demonstrate that the system can acquire high quality quantitative phase images with nanometer axial and sub-micron lateral resolution in a small form factor, making it a promising candidate for resource-limited settings and remote locations. Our design represents a significant step forward in making telecentric digital holographic microscopy accessible and affordable to the broader community.

摘要

我们展示了一种低成本、便携式远心数字全息显微镜（P-TDHM）的设计，该显微镜采用现成的组件。我们描述了系统的硬件和软件元素，并通过对从纳米打印目标到活的HeLa细胞、HEK293细胞的样本进行成像，以及通过同轴和离轴模式来评估其性能。我们的结果表明，该系统能够以小尺寸获得具有纳米轴向和亚微米横向分辨率的高质量定量相位图像，使其成为资源有限环境和偏远地区的一个有前景的候选方案。我们的设计代表了在使远心数字全息显微镜对更广泛的群体可及且价格可承受方面向前迈出的重要一步。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/927536e74b63/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/c2f3c598345e/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/75f39865c7e1/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/5b158c219250/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/fcf9323f1dfc/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/17f7f6b54eca/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/33be58838861/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/cc0198e05d97/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/f9da89ab9fa1/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/927536e74b63/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/c2f3c598345e/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/75f39865c7e1/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/5b158c219250/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/fcf9323f1dfc/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/17f7f6b54eca/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/33be58838861/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/cc0198e05d97/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/f9da89ab9fa1/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29c4/10847153/927536e74b63/gr5.jpg

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Commun Chem. 2021 Dec 8;4(1):170. doi: 10.1038/s42004-021-00609-9.
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Appl Opt. 2022 Dec 10;61(35):10490-10498. doi: 10.1364/AO.476308.
3
COVID-19 detection from red blood cells using highly comparative time-series analysis (HCTSA) in digital holographic microscopy.
利用数字全息显微镜中的高度比较时间序列分析（HCTSA）从红细胞中检测 COVID-19。
Opt Express. 2022 Jan 17;30(2):1723-1736. doi: 10.1364/OE.442321.
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Phase curvature compensation in digital holographic microscopy based on phase gradient fitting and optimization.
J Opt Soc Am A Opt Image Sci Vis. 2019 Dec 1;36(12):D1-D6. doi: 10.1364/JOSAA.36.0000D1.
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Evaluation of holographic imaging cytometer holomonitor M4® motility applications.评估全息成像细胞仪 holomonitor M4® 运动学应用。
Cytometry A. 2018 Nov;93(11):1125-1131. doi: 10.1002/cyto.a.23635. Epub 2018 Oct 21.
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Tomographic flow cytometry by digital holography.基于数字全息术的断层流式细胞术
Light Sci Appl. 2017 Apr 7;6(4):e16241. doi: 10.1038/lsa.2016.241. eCollection 2017 Apr.
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Appl Opt. 2018 Jan 1;57(1):A197-A204. doi: 10.1364/AO.57.00A197.
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Quantitative phase imaging for cell culture quality control.用于细胞培养质量控制的定量相成像
Cytometry A. 2017 May;91(5):470-481. doi: 10.1002/cyto.a.23082. Epub 2017 Mar 6.
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Automatic full compensation of quantitative phase imaging in off-axis digital holographic microscopy.离轴数字全息显微镜中定量相位成像的自动全补偿
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