Tsai Ching-Cheng, Chandel Arvind K Singh, Mitsuhashi Kento, Fujiyabu Takeshi, Inagaki Natsuko F, Ito Taichi
Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Department of Chemical System Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Biomacromolecules. 2024 Feb 12;25(2):1084-1095. doi: 10.1021/acs.biomac.3c01117. Epub 2024 Jan 30.
Benzaldehyde-conjugated chitosan (CH-CBA) was synthesized by a coupling reaction between chitosan (CH) and carboxybenzaldehyde (CBA). The pH-sensitive self-cross-linking can be achieved through the Schiff base reaction. The degree of substitution (DS) of CH-CBA was controlled at 1.4-12.7% by optimizing the pH and reagent stoichiometry. The dynamic Schiff base linkages conferred strong shear-thinning and self-healing properties to the hydrogels. The viscosity of the 2 wt/v % CH-CBA hydrogel decreased from 5.3 × 10 mPa·s at a shear rate of 10 s to 2.0 × 10 mPa·s at 10 s at pH 7.4. The CH-CBA hydrogel exhibited excellent biocompatibility in vitro and in vivo. Moreover, the hydrogel adhered strongly to porcine small intestine, colon, and cecum samples, comparable to commercial fibrin glue, and exhibited effective in vivo tissue sealing in a mouse cecal ligation and puncture model, highlighting its potential as a biomaterial for application in tissue adhesives, tissue engineering scaffolds, etc.
苯甲醛共轭壳聚糖（CH-CBA）是通过壳聚糖（CH）与羧基苯甲醛（CBA）之间的偶联反应合成的。通过席夫碱反应可实现pH敏感的自交联。通过优化pH值和试剂化学计量比，将CH-CBA的取代度（DS）控制在1.4-12.7%。动态席夫碱键赋予水凝胶强大的剪切变稀和自愈性能。在pH 7.4条件下，2 wt/v% CH-CBA水凝胶的粘度在剪切速率为10 s时从5.3×10 mPa·s降至10 s时的2.0×10 mPa·s。CH-CBA水凝胶在体外和体内均表现出优异的生物相容性。此外，该水凝胶与猪小肠、结肠和盲肠样本的粘附力很强，与市售纤维蛋白胶相当，并且在小鼠盲肠结扎和穿刺模型中表现出有效的体内组织密封性能，突出了其作为生物材料在组织粘合剂、组织工程支架等方面应用的潜力。
