Sularsih Sularsih, Fransiska Wanli, Salsabila Syifa, Rahmitasari Fitria, Soesilo Diana, Prananingrum Widyasri
Department of Dental Materials, Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia.
Faculty of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia.
Eur J Dent. 2024 Oct;18(4):1135-1141. doi: 10.1055/s-0044-1782212. Epub 2024 May 2.
The aim of this research was to analyze expression of vascular endothelial growth factor (VEGF), blood vessels, and fibroblast cell proliferation in direct pulp capping treatment of using a combination of chitosan and hydroxyapatite paste.
The samples were male strains of Wistar rats, weighing 200 to 250 g and aged between 8 and 16 weeks. The occlusal surface of the molars of was prepared with class I cavity and then perforated with the tip of an explorer. Sixty male rats were divided into the following: control group (KA) in which the cavity was filled with glass ionomer cement; control group (KB), in which the cavity was filled with Ca(OH)2; PA group, in which the cavity was filled with chitosan (CH); PB group, in which the cavity was filled with hydroxyapatite (HA); and PC group, in which the cavity was filled with chitosan and hydroxyapatite (CH-HA). Each group was divided into 3-, 7-, and 14-day observation groups. The mandibular bone of the molar was cut and histopathological examination was performed to analyze the blood vessels and fibroblast cell proliferation. Immunohistochemistry examination was done to examine the expression of VEGF.
The data variation was analyzed with One Way Analysis of Variance (ANOVA) test and continued with multiple comparison Least Significant (LSD) test to determine the different pairs of group.
Analysis of variance (ANOVA) showed a significant increase in the expression of VEGF, blood vessels, and fibroblast cell proliferations ( ≤ 0.05;), especially in the PC group compared to the other four groups. The least significant test (LSD) test showed significant differences between the groups on the expression of VEGF, blood vessels, and fibroblast cell proliferations.
The combination of chitosan and hydroxyapatite could promote healing of direct pulp capping treatment by increasing the expression of VEGF, blood vessel, and fibroblast cell proliferation.
本研究旨在分析壳聚糖与羟基磷灰石糊剂联合应用于直接盖髓治疗时血管内皮生长因子（VEGF）的表达、血管及成纤维细胞增殖情况。
样本为体重200至250克、年龄8至16周的雄性Wistar大鼠。制备大鼠磨牙咬合面的I类洞，然后用探针尖端穿孔。60只雄性大鼠分为以下几组：对照组（KA），洞腔用玻璃离子水门汀充填；对照组（KB），洞腔用氢氧化钙充填；PA组，洞腔用壳聚糖（CH）充填；PB组，洞腔用羟基磷灰石（HA）充填；PC组，洞腔用壳聚糖和羟基磷灰石（CH-HA）充填。每组再分为3天、7天和14天观察组。切取磨牙的下颌骨进行组织病理学检查，以分析血管和成纤维细胞增殖情况。进行免疫组织化学检查以检测VEGF的表达。
采用单因素方差分析（ANOVA）检验分析数据变异情况，并继续进行多重比较最小显著差（LSD）检验以确定不同组对之间的差异。
方差分析（ANOVA）显示，VEGF表达、血管及成纤维细胞增殖均显著增加（P≤0.05），尤其是PC组与其他四组相比。最小显著差（LSD）检验显示，各组在VEGF表达、血管及成纤维细胞增殖方面存在显著差异。
壳聚糖与羟基磷灰石联合应用可通过增加VEGF表达、血管生成及成纤维细胞增殖促进直接盖髓治疗的愈合。
