Hendrijantini Nike, Rostiny Rostiny, Kuntjoro Mefina, Sidharta Kent, Wiyono Dea Syarafina Putri, Anindyanari Alocitta, Salim Sherman
Department of Prosthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
Department of Undergraduate student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
Contemp Clin Dent. 2018 Oct-Dec;9(4):582-586. doi: 10.4103/ccd.ccd_617_18.
Prolongation of the inflammatory process in hyperglycemic interferes with bone formation, inhibits the healing process, and triggers bone resorption. A combination of spirulina and chitosan in the tooth socket of is expected to promote the bone remodeling process. This study aimed to evaluate the effect of spirulina and chitosan on angiogenesis, osteoclast, and osteoblast cell in tooth socket models of type 1 diabetes.
A laboratory-based experiment involving 36 , divided into three groups (nondiabetes mellitus (DM), uncontrolled DM, and controlled DM) and further divided into six subgroups. The controlled groups (K1, K2, and K3) were induced with 3% carboxymethyl cellulose Na, while the treated groups were induced with 12% spirulina and 20% chitosan. On the 14 day, the mandibles of the rats were removed. The capillary lumen, osteoblasts, and osteoclast cells were counted by hypothalamic-pituitary-adrenal examination and the results analyzed by means of Shapiro-Wilk, Levene's, one-way ANOVA, and Tukey's honestly significant difference test.
There was a significant increment in the number of capillary lumen, osteoblast cells, and a decrease in osteoclasts in all three treated groups (P1, P2, and P3).
A combination of spirulina and chitosan can effectively promote the healing process in postextraction sockets of type 1 DM .
高血糖状态下炎症过程的延长会干扰骨形成，抑制愈合过程，并引发骨吸收。螺旋藻和壳聚糖联合应用于牙槽窝有望促进骨重塑过程。本研究旨在评估螺旋藻和壳聚糖对1型糖尿病牙槽窝模型中血管生成、破骨细胞和成骨细胞的影响。
一项基于实验室的实验，涉及36只……，分为三组（非糖尿病、未控制的糖尿病和控制良好的糖尿病），并进一步分为六个亚组。对照组（K1、K2和K3）用3%羧甲基纤维素钠诱导，而治疗组用12%螺旋藻和20%壳聚糖诱导。在第14天，取出大鼠的下颌骨。通过下丘脑-垂体-肾上腺检查对毛细血管腔、成骨细胞和破骨细胞进行计数，并采用Shapiro-Wilk检验、Levene检验、单因素方差分析和Tukey真实显著性差异检验对结果进行分析。
所有三个治疗组（P1、P2和P3）的毛细血管腔数量、成骨细胞数量均显著增加，破骨细胞数量减少。
螺旋藻和壳聚糖联合应用可有效促进1型糖尿病拔牙后牙槽窝的愈合过程。
