Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand.
Functional Ingredients and Food Innovation Research Group (IFIG), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006351.
Four newly discovered Gram-stain-negative bacteria, designated as BL00010, BL00058, D8-11 and BL00200, were isolated from water samples collected at three hydrological monitoring stations (namely Chiang Saen, Chiang Khan and Nong Khai) located along the Mekong River in Thailand. An investigation encompassing phenotypic, chemotaxonomic and genomic traits was conducted. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that all four isolates represented members of the genus . These isolates were closely related to KCTC 62564 with a similarity of 99.59%. The major fatty acids of the four novel isolates included C and Cω7 and/or Cω6, whereas the major respiratory quinone was identified as ubiquinone Q-8. In addition, phosphatidylethanolamine was identified as a major polar lipid in these bacteria. The genomes of BL00010, BL00058, D8-11 and BL00200 were similar in size (3.88-4.01 Mbp) and DNA G+C contents (59.5, 59.3, 59.5 and 59.3 mol%, respectively). In contrast to KCTC 62564 and KCTC 32394, the newly discovered species possessed several genes involved in nitrite and nitrile metabolism, which may be related to their unique adaptation to nitrile-rich environments. From the results of the pairwise analysis of average nucleotide identity of the whole genome and digital DNA-DNA hybridisation, it was evident that BL00010 and D8-11 represented two novel species, for which we propose the nomenclature sp. nov., with the type strain BL00010 (TBRC 17198 = NBRC 116413), and sp. nov., with the type strain D8-11 (TBRC 17307 = NBRC 116415).
从泰国湄公河上的三个水文监测站（清盛、清孔和廊开）采集的水样中分离到四株新的革兰氏阴性菌，分别命名为 BL00010、BL00058、D8-11 和 BL00200。对这些菌株进行了表型、化学分类和基因组特征的研究。基于 16S rRNA 基因序列的系统发育分析结果表明，这 4 株菌均为 属的成员。这些菌株与 KCTC 62564 的相似性最高，为 99.59%。四个新分离株的主要脂肪酸包括 C 和 Cω7 和/或 Cω6，而主要的呼吸醌是泛醌 Q-8。此外，磷脂酰乙醇胺被鉴定为这些细菌的主要极性脂。BL00010、BL00058、D8-11 和 BL00200 的基因组大小（3.88-4.01 Mbp）和 DNA G+C 含量（分别为 59.5%、59.3%、59.5%和 59.3%mol%）相似。与 KCTC 62564 和 KCTC 32394 不同，新发现的物种具有一些参与亚硝酸盐和腈代谢的基因，这可能与它们对富含腈的环境的独特适应有关。从全基因组平均核苷酸同一性和数字 DNA-DNA 杂交的成对分析结果来看，BL00010 和 D8-11 代表两个新的种，我们建议将其命名为 sp. nov.，其模式菌株为 BL00010（TBRC 17198=NBRC 116413），以及 sp. nov.，其模式菌株为 D8-11（TBRC 17307=NBRC 116415）。
