Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Oct;69(10):3135-3140. doi: 10.1099/ijsem.0.003602.
A Gram-staining-negative, aerobic, motile with a single polar flagellum and rod-shaped bacterium as a bacterial host of podovirus P26218, designated IMCC26218, was isolated from Lake Soyang, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC26218 belonged to the genus of the family and shared 97.7-99.0 % sequence similarities with species. The draft whole genome sequence of strain IMCC26218 was ca. 4.9 Mbp in size with the DNA G+C content of 62.3 mol%. Average nucleotide identity (ANI) and digital DNA-DNA hybridisation (dDDH) values between strain IMCC26218 and other were 74.0-77.3 % and 19.5-21.0 %, respectively, showing that the strain represents a new species. The strain contained summed feature 3 (C 6 and/or C 7) and C as the major fatty acids and phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminolipids and two unidentified lipids as major polar lipids. The predominant isoprenoid quinone of the strain was ubiquinone-8 (Q-8). On the basis of the phylogenetic and phenotypic characteristics, strain IMCC26218 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMCC26218 (=KACC 18983=NBRC 112709).
一株革兰氏阴性、需氧、可运动的杆菌，具有单极鞭毛，是 podovirus P26218 的细菌宿主，从韩国Soyang 湖分离得到，命名为 IMCC26218。基于 16S rRNA 基因序列的系统发育分析显示，菌株 IMCC26218 属于 科的 属，与 种的序列相似性为 97.7-99.0%。菌株 IMCC26218 的全基因组草图大小约为 4.9 Mbp，GC 含量为 62.3 mol%。与其他 种的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 74.0-77.3%和 19.5-21.0%，表明该菌株代表了一个新的 种。该菌株含有 summed feature 3（C 6 和/或 C 7）和 C 作为主要脂肪酸和磷脂酰乙醇胺，三种未鉴定的磷脂、两种未鉴定的氨基醇和两种未鉴定的脂质作为主要极性脂质。该菌株的主要类异戊二烯醌为 ubiquinone-8 (Q-8)。基于系统发育和表型特征，菌株 IMCC26218 被认为代表了 属的一个新种，建议将其命名为 sp. nov.。模式菌株为 IMCC26218（=KACC 18983=NBRC 112709）。
