Kato A, Takaiwa F, Shinozaki K, Sugiura M
National Institute of Agrobiological Resources, Tsukuba, Japan.
Curr Genet. 1985;9(5):405-9. doi: 10.1007/BF00421612.
The location and nucleotide sequence of the genes and flanking regions for tRNAArg(ACG) and tRNALeu(UAG) on tobacco chloroplast DNA have been determined. The gene arrangement is 5S rRNA-260 bp-tRNAArg-581 bp-tRNAAsn-5.2 kbp-tRNALeu. The tRNAArg and tRNALeu genes are expressed in the chloroplasts. The opposite strand of the tRNAArg gene contains a tRNAArg-like sequence. The tRNAArg, tRNAAsn and tRNALeu coding regions are contained in open reading frames.
烟草叶绿体DNA上tRNAArg(ACG)和tRNALeu(UAG)基因及其侧翼区域的位置和核苷酸序列已被确定。基因排列为5S rRNA - 260 bp - tRNAArg - 581 bp - tRNAAsn - 5.2 kbp - tRNALeu。tRNAArg和tRNALeu基因在叶绿体中表达。tRNAArg基因的互补链包含一个类似tRNAArg的序列。tRNAArg、tRNAAsn和tRNALeu编码区包含在开放阅读框中。