Department of Biology, Faculty of Science, Nagoya University, Chikusa, Nagoya 464, Japan.
Proc Natl Acad Sci U S A. 1984 Jan;81(2):405-8. doi: 10.1073/pnas.81.2.405.
The nucleotide sequences of tobacco chloroplast genes for tRNA(Gly) (UCC) and tRNA(Arg) (UCU) have been determined. The tRNA(Gly) gene has a 691-base-pair intron located in the D stem while the tRNA(Arg) gene does not have any intron. The tRNA(Gly) and tRNA(Arg) genes are encoded on the same strand and separated by a 169-base-pair spacer. The tRNA(Gly) gene is transcribed as a 900-base precursor RNA molecule. The tRNA(Gly) and tRNA(Arg) deduced from the DNA sequences show 84% and 55% sequence homologies with Escherichia coli tRNA(Gly) (UCC) and phage T4 tRNA(Arg) (UCU), respectively.
已测定烟草叶绿体基因 tRNA(Gly)(UCC)和 tRNA(Arg)(UCU)的核苷酸序列。tRNA(Gly)基因有一个 691 碱基对的内含子位于 D 茎，而 tRNA(Arg)基因没有任何内含子。tRNA(Gly)和 tRNA(Arg)基因编码在同一链上，由 169 碱基对的间隔区分开。tRNA(Gly)基因作为一个 900 碱基的前体 RNA 分子转录。从 DNA 序列推断的 tRNA(Gly)和 tRNA(Arg)与大肠杆菌 tRNA(Gly)(UCC)和噬菌体 T4 tRNA(Arg)(UCU)分别具有 84%和 55%的序列同源性。
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