Bonnard G, Weil J H, Steinmetz A
Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., Université Louis Pasteur, Strasbourg, France.
Curr Genet. 1985;9(5):417-22. doi: 10.1007/BF00421614.
A cluster of three tRNA genes located on fragment Bam6a from Vicia faba chloroplast DNA has been sequenced: it contains the genes for tRNA(CAALeu), tRNA(UAALeu) and tRNA(Phe). The two tRNA(Leu) genes are separated by 443 bp and are transcribed divergently from different DNA strands. The intergenic region contains a series of short repeats and a partial copy of the split tRNA(UAALeu) gene which includes 100 bp of the 5' flanking region, 35 bp of the 5'exon and the first 42 bp of the intron. It is possible that some of these duplications occurred upon the rearrangement of the two tRNA(Leu) genes in broad bean (and in pea) or upon the deletion of one copy of the inverted repeat, since in all other higher plant chloroplast genomes studied so far these two tRNA(Leu) genes are located far apart on the genome, one being in the inverted repeat region, the other one in the large single copy region. The tRNA(Phe) and tRNA(UAALeu) are encoded by the same DNA strand, and separated by 110 bp.
对蚕豆叶绿体DNA中Bam6a片段上的一组三个tRNA基因进行了测序：它包含tRNA（CAALeu）、tRNA（UAALeu）和tRNA（Phe）的基因。两个tRNA（Leu）基因相隔443 bp，从不同的DNA链上反向转录。基因间区域包含一系列短重复序列和分裂的tRNA（UAALeu）基因的部分拷贝，其中包括5'侧翼区域的100 bp、5'外显子的35 bp和内含子的前42 bp。这些重复中的一些可能是在蚕豆（和豌豆）中两个tRNA（Leu）基因重排时或一个反向重复拷贝缺失时发生的，因为在迄今为止研究的所有其他高等植物叶绿体基因组中，这两个tRNA（Leu）基因在基因组上相距很远，一个位于反向重复区域，另一个位于大单拷贝区域。tRNA（Phe）和tRNA（UAALeu）由同一条DNA链编码，相隔110 bp。
